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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MLH1 All Species: 6.06
Human Site: S466 Identified Species: 11.11
UniProt: P40692 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.25
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P40692 NP_000240.1 756 84601 S466 S E K R G P T S S N P R K R H
Chimpanzee Pan troglodytes XP_001170274 756 84655 S466 S E K R G P T S S N P R K R H
Rhesus Macaque Macaca mulatta XP_001087274 583 64794 N306 S P Q N V D V N V H P T K H E
Dog Lupus familis XP_534219 757 84567 P467 K K G P P S S P G N P R K R P
Cat Felis silvestris
Mouse Mus musculus Q9JK91 760 84662 P470 K A A P T S S P G S S R K R H
Rat Rattus norvegicus P97679 757 84522 P468 P Q R H P S S P G S S R K R H
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus XP_418828 757 84832 E467 K S G H L S P E K A L P R K R
Frog Xenopus laevis NP_001090545 750 84121 E462 D D S P N N I E Q P R K R P R
Zebra Danio Brachydanio rerio NP_956953 724 80793 D447 T D A Q P P G D E A P P R K R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_001120100 716 81818 D436 C L T P N V I D I Q Q K D K S
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_793318 745 83320 R455 F L C R K R P R S E V S S D T
Poplar Tree Populus trichocarpa XP_002325965 747 83627 S448 S L A A V R S S V R Q R R N P
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P38920 769 87044 Q474 K D Q P K K K Q K L G D Y K V
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 76 93 N.A. 88.1 87 N.A. N.A. 80 75.1 69.7 N.A. N.A. 50.6 N.A. 60.3
Protein Similarity: 100 99.8 76.5 96.1 N.A. 93.9 93.3 N.A. N.A. 88.1 85.9 81 N.A. N.A. 70.7 N.A. 75.7
P-Site Identity: 100 100 20 33.3 N.A. 26.6 26.6 N.A. N.A. 0 0 13.3 N.A. N.A. 0 N.A. 13.3
P-Site Similarity: 100 100 40 46.6 N.A. 40 53.3 N.A. N.A. 13.3 20 46.6 N.A. N.A. 13.3 N.A. 13.3
Percent
Protein Identity: 34.7 N.A. N.A. N.A. 36 N.A.
Protein Similarity: 56.6 N.A. N.A. N.A. 58.5 N.A.
P-Site Identity: 20 N.A. N.A. N.A. 0 N.A.
P-Site Similarity: 33.3 N.A. N.A. N.A. 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 24 8 0 0 0 0 0 16 0 0 0 0 0 % A
% Cys: 8 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 8 24 0 0 0 8 0 16 0 0 0 8 8 8 0 % D
% Glu: 0 16 0 0 0 0 0 16 8 8 0 0 0 0 8 % E
% Phe: 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 16 0 16 0 8 0 24 0 8 0 0 0 0 % G
% His: 0 0 0 16 0 0 0 0 0 8 0 0 0 8 31 % H
% Ile: 0 0 0 0 0 0 16 0 8 0 0 0 0 0 0 % I
% Lys: 31 8 16 0 16 8 8 0 16 0 0 16 47 31 0 % K
% Leu: 0 24 0 0 8 0 0 0 0 8 8 0 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 8 16 8 0 8 0 24 0 0 0 8 0 % N
% Pro: 8 8 0 39 24 24 16 24 0 8 39 16 0 8 16 % P
% Gln: 0 8 16 8 0 0 0 8 8 8 16 0 0 0 0 % Q
% Arg: 0 0 8 24 0 16 0 8 0 8 8 47 31 39 24 % R
% Ser: 31 8 8 0 0 31 31 24 24 16 16 8 8 0 8 % S
% Thr: 8 0 8 0 8 0 16 0 0 0 0 8 0 0 8 % T
% Val: 0 0 0 0 16 8 8 0 16 0 8 0 0 0 8 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _