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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MTHFR All Species: 40
Human Site: S61 Identified Species: 62.86
UniProt: P42898 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P42898 NP_005948.3 656 74597 S61 E S G D K W F S L E F F P P R
Chimpanzee Pan troglodytes XP_001141362 697 78964 S102 E S G D K W F S L E F F P P R
Rhesus Macaque Macaca mulatta XP_001105188 697 79008 S102 E S G D K W F S L E F F P P R
Dog Lupus familis XP_535405 780 87445 S103 E S G D R W F S L E F F P P R
Cat Felis silvestris
Mouse Mus musculus Q9WU20 654 74631 S60 D S G D K W F S L E F F P P R
Rat Rattus norvegicus XP_342976 695 78719 S101 D S G D K W F S L E F F P P R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516302 646 73079 S58 D S G D K W F S L E F F P P R
Chicken Gallus gallus XP_417645 508 58330
Frog Xenopus laevis NP_001080092 643 73624 S55 E A G D R W F S L E F F P P R
Zebra Danio Brachydanio rerio NP_001121727 656 74637 S61 E S G D H W F S L E F F P P R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q17693 663 75468 S74 D E K Q Q F F S L E F F P P R
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa XP_002310366 608 67799 A57 E R M D R M V A H N P S F C D
Maize Zea mays Q9SE94 593 66410 G45 M D R M V A H G P S F C D I T
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9SE60 592 66270 Y44 R M D R L V S Y G P T F C D I
Baker's Yeast Sacchar. cerevisiae P53128 600 68542 D52 A S L P Q F I D I T W N A G G
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 93.9 91.9 76.4 N.A. 89.9 83.8 N.A. 85 64.9 79.2 77.2 N.A. N.A. N.A. 46.4 N.A.
Protein Similarity: 100 94.1 93.1 80.7 N.A. 95.1 89.7 N.A. 92.5 71.4 88.5 87.5 N.A. N.A. N.A. 67.7 N.A.
P-Site Identity: 100 100 100 93.3 N.A. 93.3 93.3 N.A. 93.3 0 86.6 93.3 N.A. N.A. N.A. 60 N.A.
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 100 0 100 93.3 N.A. N.A. N.A. 80 N.A.
Percent
Protein Identity: 43.4 41.7 N.A. 41 38.7 N.A.
Protein Similarity: 60 59.1 N.A. 58.8 57.3 N.A.
P-Site Identity: 13.3 6.6 N.A. 6.6 6.6 N.A.
P-Site Similarity: 26.6 6.6 N.A. 6.6 33.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 7 0 0 0 7 0 7 0 0 0 0 7 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 7 7 7 0 % C
% Asp: 27 7 7 67 0 0 0 7 0 0 0 0 7 7 7 % D
% Glu: 47 7 0 0 0 0 0 0 0 67 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 14 67 0 0 0 74 74 7 0 0 % F
% Gly: 0 0 60 0 0 0 0 7 7 0 0 0 0 7 7 % G
% His: 0 0 0 0 7 0 7 0 7 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 7 0 7 0 0 0 0 7 7 % I
% Lys: 0 0 7 0 40 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 0 0 7 0 7 0 0 0 67 0 0 0 0 0 0 % L
% Met: 7 7 7 7 0 7 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 0 0 7 0 7 0 0 0 % N
% Pro: 0 0 0 7 0 0 0 0 7 7 7 0 67 67 0 % P
% Gln: 0 0 0 7 14 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 7 7 7 7 20 0 0 0 0 0 0 0 0 0 67 % R
% Ser: 0 60 0 0 0 0 7 67 0 7 0 7 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 0 0 7 7 0 0 0 7 % T
% Val: 0 0 0 0 7 7 7 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 60 0 0 0 0 7 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _