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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NSF All Species: 13.64
Human Site: T46 Identified Species: 21.43
UniProt: P46459 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P46459 NP_006169.2 744 82594 T46 T S P N H R Y T F T L K T H P
Chimpanzee Pan troglodytes XP_511626 842 92301 T119 T S P N H R Y T F T L K T H P
Rhesus Macaque Macaca mulatta XP_001105450 854 93325 T156 T S P N H R Y T F T L K T H P
Dog Lupus familis XP_548044 752 83541 T46 T S P N H R Y T F T L R T H P
Cat Felis silvestris
Mouse Mus musculus P46460 744 82595 I46 T S P N H K Y I F T L R T H P
Rat Rattus norvegicus Q9QUL6 744 82634 I46 T S P N H K Y I F T L R T H P
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus XP_418094 740 82158 I46 T S P N H K Y I F T L R T H P
Frog Xenopus laevis P23787 805 89193 M46 V S L S Q A K M D E L Q L F R
Zebra Danio Brachydanio rerio Q7ZU99 806 89405 M46 V S L S Q A K M D E L Q L F R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P46461 745 82537 I46 P A P G Q H F I F A L E K T V
Honey Bee Apis mellifera XP_001120201 743 82591 V47 T A P N H H F V F T V R Q H H
Nematode Worm Caenorhab. elegans Q94392 824 91316 I110 P G P A H H Y I F S I R N D G
Sea Urchin Strong. purpuratus NP_999752 746 82671 V48 A I P G P S Y V F A T V A H P
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9M0Y8 742 81469 I55 A N V A D S F I L S L C G H G
Baker's Yeast Sacchar. cerevisiae P18759 758 84038 V65 I I I D N L F V F T T R H S N
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 88 86.8 98.1 N.A. 98.1 97.8 N.A. N.A. 93.6 26.2 25 N.A. 62.4 65.8 50.6 64.8
Protein Similarity: 100 88.2 87.1 98.8 N.A. 99.7 99.7 N.A. N.A. 96.5 44.2 44.4 N.A. 77.7 81.1 66.2 80.5
P-Site Identity: 100 100 100 93.3 N.A. 80 80 N.A. N.A. 80 13.3 13.3 N.A. 20 46.6 26.6 33.3
P-Site Similarity: 100 100 100 100 N.A. 93.3 93.3 N.A. N.A. 93.3 26.6 26.6 N.A. 40 73.3 46.6 33.3
Percent
Protein Identity: N.A. N.A. N.A. 43.5 44.9 N.A.
Protein Similarity: N.A. N.A. N.A. 64.6 64.2 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 33.3 40 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 14 14 0 14 0 14 0 0 0 14 0 0 7 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 % C
% Asp: 0 0 0 7 7 0 0 0 14 0 0 0 0 7 0 % D
% Glu: 0 0 0 0 0 0 0 0 0 14 0 7 0 0 0 % E
% Phe: 0 0 0 0 0 0 27 0 80 0 0 0 0 14 0 % F
% Gly: 0 7 0 14 0 0 0 0 0 0 0 0 7 0 14 % G
% His: 0 0 0 0 60 20 0 0 0 0 0 0 7 67 7 % H
% Ile: 7 14 7 0 0 0 0 40 0 0 7 0 0 0 0 % I
% Lys: 0 0 0 0 0 20 14 0 0 0 0 20 7 0 0 % K
% Leu: 0 0 14 0 0 7 0 0 7 0 74 0 14 0 0 % L
% Met: 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0 % M
% Asn: 0 7 0 54 7 0 0 0 0 0 0 0 7 0 7 % N
% Pro: 14 0 74 0 7 0 0 0 0 0 0 0 0 0 54 % P
% Gln: 0 0 0 0 20 0 0 0 0 0 0 14 7 0 0 % Q
% Arg: 0 0 0 0 0 27 0 0 0 0 0 47 0 0 14 % R
% Ser: 0 60 0 14 0 14 0 0 0 14 0 0 0 7 0 % S
% Thr: 54 0 0 0 0 0 0 27 0 60 14 0 47 7 0 % T
% Val: 14 0 7 0 0 0 0 20 0 0 7 7 0 0 7 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 60 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _