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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
NSF
All Species:
36.06
Human Site:
T48
Identified Species:
56.67
UniProt:
P46459
Number Species:
14
Phosphosite Substitution
Charge Score:
0.14
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
P46459
NP_006169.2
744
82594
T48
P
N
H
R
Y
T
F
T
L
K
T
H
P
S
V
Chimpanzee
Pan troglodytes
XP_511626
842
92301
T121
P
N
H
R
Y
T
F
T
L
K
T
H
P
S
V
Rhesus Macaque
Macaca mulatta
XP_001105450
854
93325
T158
P
N
H
R
Y
T
F
T
L
K
T
H
P
S
V
Dog
Lupus familis
XP_548044
752
83541
T48
P
N
H
R
Y
T
F
T
L
R
T
H
P
S
V
Cat
Felis silvestris
Mouse
Mus musculus
P46460
744
82595
T48
P
N
H
K
Y
I
F
T
L
R
T
H
P
S
V
Rat
Rattus norvegicus
Q9QUL6
744
82634
T48
P
N
H
K
Y
I
F
T
L
R
T
H
P
S
V
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
Chicken
Gallus gallus
XP_418094
740
82158
T48
P
N
H
K
Y
I
F
T
L
R
T
H
P
S
V
Frog
Xenopus laevis
P23787
805
89193
E48
L
S
Q
A
K
M
D
E
L
Q
L
F
R
G
D
Zebra Danio
Brachydanio rerio
Q7ZU99
806
89405
E48
L
S
Q
A
K
M
D
E
L
Q
L
F
R
G
D
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
P46461
745
82537
A48
P
G
Q
H
F
I
F
A
L
E
K
T
V
E
V
Honey Bee
Apis mellifera
XP_001120201
743
82591
T49
P
N
H
H
F
V
F
T
V
R
Q
H
H
E
V
Nematode Worm
Caenorhab. elegans
Q94392
824
91316
S112
P
A
H
H
Y
I
F
S
I
R
N
D
G
S
I
Sea Urchin
Strong. purpuratus
NP_999752
746
82671
A50
P
G
P
S
Y
V
F
A
T
V
A
H
P
D
L
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Q9M0Y8
742
81469
S57
V
A
D
S
F
I
L
S
L
C
G
H
G
S
I
Baker's Yeast
Sacchar. cerevisiae
P18759
758
84038
T67
I
D
N
L
F
V
F
T
T
R
H
S
N
D
I
Red Bread Mold
Neurospora crassa
Conservation
Percent
Protein Identity:
100
88
86.8
98.1
N.A.
98.1
97.8
N.A.
N.A.
93.6
26.2
25
N.A.
62.4
65.8
50.6
64.8
Protein Similarity:
100
88.2
87.1
98.8
N.A.
99.7
99.7
N.A.
N.A.
96.5
44.2
44.4
N.A.
77.7
81.1
66.2
80.5
P-Site Identity:
100
100
100
93.3
N.A.
80
80
N.A.
N.A.
80
6.6
6.6
N.A.
26.6
46.6
33.3
33.3
P-Site Similarity:
100
100
100
100
N.A.
93.3
93.3
N.A.
N.A.
93.3
20
20
N.A.
40
66.6
60
40
Percent
Protein Identity:
N.A.
N.A.
N.A.
43.5
44.9
N.A.
Protein Similarity:
N.A.
N.A.
N.A.
64.6
64.2
N.A.
P-Site Identity:
N.A.
N.A.
N.A.
20
13.3
N.A.
P-Site Similarity:
N.A.
N.A.
N.A.
40
46.6
N.A.
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
0
14
0
14
0
0
0
14
0
0
7
0
0
0
0
% A
% Cys:
0
0
0
0
0
0
0
0
0
7
0
0
0
0
0
% C
% Asp:
0
7
7
0
0
0
14
0
0
0
0
7
0
14
14
% D
% Glu:
0
0
0
0
0
0
0
14
0
7
0
0
0
14
0
% E
% Phe:
0
0
0
0
27
0
80
0
0
0
0
14
0
0
0
% F
% Gly:
0
14
0
0
0
0
0
0
0
0
7
0
14
14
0
% G
% His:
0
0
60
20
0
0
0
0
0
0
7
67
7
0
0
% H
% Ile:
7
0
0
0
0
40
0
0
7
0
0
0
0
0
20
% I
% Lys:
0
0
0
20
14
0
0
0
0
20
7
0
0
0
0
% K
% Leu:
14
0
0
7
0
0
7
0
74
0
14
0
0
0
7
% L
% Met:
0
0
0
0
0
14
0
0
0
0
0
0
0
0
0
% M
% Asn:
0
54
7
0
0
0
0
0
0
0
7
0
7
0
0
% N
% Pro:
74
0
7
0
0
0
0
0
0
0
0
0
54
0
0
% P
% Gln:
0
0
20
0
0
0
0
0
0
14
7
0
0
0
0
% Q
% Arg:
0
0
0
27
0
0
0
0
0
47
0
0
14
0
0
% R
% Ser:
0
14
0
14
0
0
0
14
0
0
0
7
0
60
0
% S
% Thr:
0
0
0
0
0
27
0
60
14
0
47
7
0
0
0
% T
% Val:
7
0
0
0
0
20
0
0
7
7
0
0
7
0
60
% V
% Trp:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% W
% Tyr:
0
0
0
0
60
0
0
0
0
0
0
0
0
0
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _