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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NOTCH1 All Species: 17.58
Human Site: S2121 Identified Species: 32.22
UniProt: P46531 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P46531 NP_060087.3 2555 272505 S2121 D E Y N L V R S P Q L H G A P
Chimpanzee Pan troglodytes XP_520371 2555 272693 S2121 D E Y N L V R S P Q L H G A P
Rhesus Macaque Macaca mulatta XP_001114248 2471 265649 V2059 D R M H H D I V R L L D E Y N
Dog Lupus familis XP_537795 2544 270793 S2090 D E Y S L V R S P Q L H G P A
Cat Felis silvestris
Mouse Mus musculus Q01705 2531 271296 S2110 D E Y N L V R S P Q L H G T A
Rat Rattus norvegicus Q07008 2531 270981 S2111 D E Y N L V R S P Q L H G T A
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001512292 2467 261963 L2057 M H H D I V R L L S E Y N V A
Chicken Gallus gallus XP_415420 2590 279561 R2159 L D E Y N L V R S P P L H S G
Frog Xenopus laevis P21783 2524 275106 E2112 D I V H L L D E Y N L V K S P
Zebra Danio Brachydanio rerio P46530 2437 262289 S2015 D P N A V D D S G K S A L H W
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P07207 2703 288835 P2144 D E H V P R S P Q M L S M T P
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P14585 1429 157097 E1021 I P T E P E P E S P I K L H T
Sea Urchin Strong. purpuratus P10079 1064 112055 C656 D Q V N S Y V C F C L P G F T
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.7 53.1 89.5 N.A. 89.3 90.2 N.A. 51.3 79.6 74.3 67.7 N.A. 44.4 N.A. 20.5 21.6
Protein Similarity: 100 99.8 66.5 93.7 N.A. 93 94 N.A. 63.6 87.6 85.2 78.7 N.A. 58.6 N.A. 33.3 29.4
P-Site Identity: 100 100 13.3 80 N.A. 86.6 86.6 N.A. 13.3 0 26.6 13.3 N.A. 26.6 N.A. 0 26.6
P-Site Similarity: 100 100 20 86.6 N.A. 86.6 86.6 N.A. 40 20 46.6 26.6 N.A. 33.3 N.A. 6.6 33.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 8 0 0 0 0 0 0 0 8 0 16 31 % A
% Cys: 0 0 0 0 0 0 0 8 0 8 0 0 0 0 0 % C
% Asp: 77 8 0 8 0 16 16 0 0 0 0 8 0 0 0 % D
% Glu: 0 47 8 8 0 8 0 16 0 0 8 0 8 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 8 0 0 0 0 8 0 % F
% Gly: 0 0 0 0 0 0 0 0 8 0 0 0 47 0 8 % G
% His: 0 8 16 16 8 0 0 0 0 0 0 39 8 16 0 % H
% Ile: 8 8 0 0 8 0 8 0 0 0 8 0 0 0 0 % I
% Lys: 0 0 0 0 0 0 0 0 0 8 0 8 8 0 0 % K
% Leu: 8 0 0 0 47 16 0 8 8 8 70 8 16 0 0 % L
% Met: 8 0 8 0 0 0 0 0 0 8 0 0 8 0 0 % M
% Asn: 0 0 8 39 8 0 0 0 0 8 0 0 8 0 8 % N
% Pro: 0 16 0 0 16 0 8 8 39 16 8 8 0 8 31 % P
% Gln: 0 8 0 0 0 0 0 0 8 39 0 0 0 0 0 % Q
% Arg: 0 8 0 0 0 8 47 8 8 0 0 0 0 0 0 % R
% Ser: 0 0 0 8 8 0 8 47 16 8 8 8 0 16 0 % S
% Thr: 0 0 8 0 0 0 0 0 0 0 0 0 0 24 16 % T
% Val: 0 0 16 8 8 47 16 8 0 0 0 8 0 8 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 % W
% Tyr: 0 0 39 8 0 8 0 0 8 0 0 8 0 8 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _