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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: LIG4 All Species: 13.64
Human Site: Y525 Identified Species: 21.43
UniProt: P49917 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P49917 NP_001091738.1 911 103971 Y525 L G L K L A K Y W K P F H R K
Chimpanzee Pan troglodytes XP_509726 911 103942 Y525 L G L K L A K Y W K P F H R K
Rhesus Macaque Macaca mulatta XP_001084107 642 73046 V287 Q M H K D G D V Y K Y F S R N
Dog Lupus familis XP_542663 911 104163 H525 L G L K L A K H W K P F H K K
Cat Felis silvestris
Mouse Mus musculus Q8BTF7 911 104067 Y525 L G L K L A K Y W K P F H K K
Rat Rattus norvegicus NP_001099565 911 104477 Y525 L G L K L A K Y W K P F Q R K
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus Q90YB1 912 104431 H530 L G L K L A K H W K P Y N R K
Frog Xenopus laevis NP_001081114 911 104084 H530 L G L K L A P H W K T Y R K R
Zebra Danio Brachydanio rerio NP_001096593 909 103624 H530 L G V K L A K H W K V Y N K N
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_572907 918 104813 V536 L K P H W H D V V N E P P P L
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001188102 952 108709 H563 F N Q K L A K H W K K Y D K E
Poplar Tree Populus trichocarpa XP_002325262 1242 139306 P530 Y F R K N E Y P K N S P P S F
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9LL84 1219 137833 P522 Y F R K N E H P K K A P P S F
Baker's Yeast Sacchar. cerevisiae Q08387 944 108496 H559 I D R K T R G H W K R T S E V
Red Bread Mold Neurospora crassa Q7SB49 1046 118891 S603 K W Q D W D P S S P P T E Y I
Conservation
Percent
Protein Identity: 100 99.4 68.9 91 N.A. 86.9 86.1 N.A. N.A. 76.5 72.5 64.1 N.A. 29.7 N.A. N.A. 48.5
Protein Similarity: 100 99.7 69.8 95.5 N.A. 92.5 92 N.A. N.A. 87.1 84 79.3 N.A. 51.3 N.A. N.A. 65.4
P-Site Identity: 100 100 26.6 86.6 N.A. 93.3 93.3 N.A. N.A. 80 53.3 53.3 N.A. 6.6 N.A. N.A. 40
P-Site Similarity: 100 100 33.3 100 N.A. 100 93.3 N.A. N.A. 100 80 86.6 N.A. 6.6 N.A. N.A. 66.6
Percent
Protein Identity: 27.2 N.A. N.A. 27.6 25.5 29.2
Protein Similarity: 43.3 N.A. N.A. 44.9 46.9 47.2
P-Site Identity: 6.6 N.A. N.A. 13.3 20 6.6
P-Site Similarity: 6.6 N.A. N.A. 13.3 33.3 6.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 60 0 0 0 0 7 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 7 0 7 7 7 14 0 0 0 0 0 7 0 0 % D
% Glu: 0 0 0 0 0 14 0 0 0 0 7 0 7 7 7 % E
% Phe: 7 14 0 0 0 0 0 0 0 0 0 40 0 0 14 % F
% Gly: 0 54 0 0 0 7 7 0 0 0 0 0 0 0 0 % G
% His: 0 0 7 7 0 7 7 40 0 0 0 0 27 0 0 % H
% Ile: 7 0 0 0 0 0 0 0 0 0 0 0 0 0 7 % I
% Lys: 7 7 0 87 0 0 54 0 14 80 7 0 0 34 40 % K
% Leu: 60 0 47 0 60 0 0 0 0 0 0 0 0 0 7 % L
% Met: 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 7 0 0 14 0 0 0 0 14 0 0 14 0 14 % N
% Pro: 0 0 7 0 0 0 14 14 0 7 47 20 20 7 0 % P
% Gln: 7 0 14 0 0 0 0 0 0 0 0 0 7 0 0 % Q
% Arg: 0 0 20 0 0 7 0 0 0 0 7 0 7 34 7 % R
% Ser: 0 0 0 0 0 0 0 7 7 0 7 0 14 14 0 % S
% Thr: 0 0 0 0 7 0 0 0 0 0 7 14 0 0 0 % T
% Val: 0 0 7 0 0 0 0 14 7 0 7 0 0 0 7 % V
% Trp: 0 7 0 0 14 0 0 0 67 0 0 0 0 0 0 % W
% Tyr: 14 0 0 0 0 0 7 27 7 0 7 27 0 7 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _