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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: CDK9 All Species: 25.76
Human Site: Y259 Identified Species: 37.78
UniProt: P50750 Number Species: 15
    Phosphosite Substitution
    Charge Score: -0.13
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P50750 NP_001252.1 372 42778 Y259 V W P N V D N Y E L Y E K L E
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta XP_001094502 352 40482 Y239 V W P N V D N Y E L Y E K L E
Dog Lupus familis XP_548446 534 59045 Y421 V W P N V D K Y E L F E K L D
Cat Felis silvestris
Mouse Mus musculus NP_570930 372 42743 Y259 V W P N V D K Y E L F E K L E
Rat Rattus norvegicus Q641Z4 372 42743 Y259 V W P N V D K Y E L F E K L E
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001520593 314 36161 R211 Y Q K L D L P R G Q K R K V K
Chicken Gallus gallus Q5ZKN1 372 42784 Y259 V W P N V D K Y E L Y Q K L E
Frog Xenopus laevis Q4V862 376 43205 Y263 V W P N V D K Y E L Y Q K L E
Zebra Danio Brachydanio rerio NP_997756 393 45129 K280 V W P G V D K K Y E L Y Q K M
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_477226 404 46768 L291 V W P G V E E L E L Y K S I E
Honey Bee Apis mellifera XP_396015 382 43973 L265 V W P G V E N L E L F N K M D
Nematode Worm Caenorhab. elegans Q9TVL3 478 53575 M329 V W P N C V N M P L W S A M S
Sea Urchin Strong. purpuratus XP_798269 410 46418 L288 V W P G L E K L E L S N T L E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9LFT8 505 56711 M271 L W P G V S K M P W F N N F K
Baker's Yeast Sacchar. cerevisiae P23293 657 74221 L313 D W A V A R Y L P G A E L T T
Red Bread Mold Neurospora crassa Q871M9 545 61347 L274 T M P G W R T L P G G Q A F S
Conservation
Percent
Protein Identity: 100 N.A. 92.4 68.5 N.A. 98.6 98.6 N.A. 77.9 93 89.3 85.5 N.A. 66 72.7 43.3 67.3
Protein Similarity: 100 N.A. 93.5 69.4 N.A. 99.7 99.7 N.A. 80.3 95.4 94.1 89.5 N.A. 75.9 83.2 56.4 75.8
P-Site Identity: 100 N.A. 100 80 N.A. 86.6 86.6 N.A. 6.6 86.6 86.6 33.3 N.A. 53.3 53.3 40 46.6
P-Site Similarity: 100 N.A. 100 93.3 N.A. 93.3 93.3 N.A. 20 93.3 93.3 40 N.A. 73.3 80 53.3 60
Percent
Protein Identity: N.A. N.A. N.A. 36 25.2 31.7
Protein Similarity: N.A. N.A. N.A. 51 39.4 45.6
P-Site Identity: N.A. N.A. N.A. 20 13.3 6.6
P-Site Similarity: N.A. N.A. N.A. 40 13.3 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 7 0 0 0 0 0 7 0 13 0 0 % A
% Cys: 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 7 0 0 0 7 50 0 0 0 0 0 0 0 0 13 % D
% Glu: 0 0 0 0 0 19 7 0 63 7 0 38 0 0 50 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 32 0 0 13 0 % F
% Gly: 0 0 0 38 0 0 0 0 7 13 7 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 % I
% Lys: 0 0 7 0 0 0 50 7 0 0 7 7 57 7 13 % K
% Leu: 7 0 0 7 7 7 0 32 0 69 7 0 7 50 0 % L
% Met: 0 7 0 0 0 0 0 13 0 0 0 0 0 13 7 % M
% Asn: 0 0 0 50 0 0 25 0 0 0 0 19 7 0 0 % N
% Pro: 0 0 88 0 0 0 7 0 25 0 0 0 0 0 0 % P
% Gln: 0 7 0 0 0 0 0 0 0 7 0 19 7 0 0 % Q
% Arg: 0 0 0 0 0 13 0 7 0 0 0 7 0 0 0 % R
% Ser: 0 0 0 0 0 7 0 0 0 0 7 7 7 0 13 % S
% Thr: 7 0 0 0 0 0 7 0 0 0 0 0 7 7 7 % T
% Val: 75 0 0 7 69 7 0 0 0 0 0 0 0 7 0 % V
% Trp: 0 88 0 0 7 0 0 0 0 7 7 0 0 0 0 % W
% Tyr: 7 0 0 0 0 0 7 44 7 0 32 7 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _