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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PLK1 All Species: 41.21
Human Site: S254 Identified Species: 56.67
UniProt: P53350 Number Species: 16
    Phosphosite Substitution
    Charge Score: -0.13
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P53350 NP_005021.2 603 68255 S254 G K P P F E T S C L K E T Y L
Chimpanzee Pan troglodytes XP_001163623 603 68223 S254 G K P P F E T S C L K E T Y L
Rhesus Macaque Macaca mulatta XP_001092070 603 68240 S254 G K P P F E T S C L K E T Y L
Dog Lupus familis XP_547091 832 93293 S482 G K P P F E T S C L K E T Y L
Cat Felis silvestris
Mouse Mus musculus Q07832 603 68282 S254 G K P P F E T S C L K E T Y L
Rat Rattus norvegicus Q62673 603 68295 S254 G K P P F E T S C L K E T Y L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001505317 482 55166 H151 N E Y S I P K H I N P V A A S
Chicken Gallus gallus NP_001025810 595 66904 S244 G K P P F E T S C L K E T Y I
Frog Xenopus laevis P70032 598 68193 S245 G K P P F E T S C L K E T Y M
Zebra Danio Brachydanio rerio NP_001003890 595 67743 S240 G K P P F E T S C L K E T Y I
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P52304 576 66955 K226 G Q P P F E T K T L K D T Y S
Honey Bee Apis mellifera XP_396707 575 66141 S224 G K P P F E T S S L K E T Y A
Nematode Worm Caenorhab. elegans P34331 649 73615 K239 G Q P P F E S K S L E E T Y S
Sea Urchin Strong. purpuratus XP_784826 587 66895 Q228 G K P P F E T Q S L K D T Y Q
Poplar Tree Populus trichocarpa
Maize Zea mays P0C8M8 626 70215 T237 V G R P P F E T S D V K Q T Y
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9STV4 445 50410 H114 S E A R K Y F H Q L I D G V D
Baker's Yeast Sacchar. cerevisiae P32562 705 81013 Q282 L I G K P P F Q A R D V N T I
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.8 99.8 69.5 N.A. 95 94.1 N.A. 69.3 80 79.7 74.3 N.A. 50.7 51.4 49.9 59.3
Protein Similarity: 100 99.8 99.8 71 N.A. 97.6 96.8 N.A. 74.7 89.8 90.5 87.5 N.A. 69.4 69.9 66.2 76.4
P-Site Identity: 100 100 100 100 N.A. 100 100 N.A. 0 93.3 93.3 93.3 N.A. 66.6 86.6 60 73.3
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 6.6 100 100 100 N.A. 80 86.6 80 80
Percent
Protein Identity: N.A. 31.7 N.A. 26 33.9 N.A.
Protein Similarity: N.A. 50.1 N.A. 40.4 51.6 N.A.
P-Site Identity: N.A. 6.6 N.A. 6.6 0 N.A.
P-Site Similarity: N.A. 20 N.A. 20 6.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 6 0 0 0 0 0 6 0 0 0 6 6 6 % A
% Cys: 0 0 0 0 0 0 0 0 53 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 6 6 18 0 0 6 % D
% Glu: 0 12 0 0 0 77 6 0 0 0 6 65 0 0 0 % E
% Phe: 0 0 0 0 77 6 12 0 0 0 0 0 0 0 0 % F
% Gly: 77 6 6 0 0 0 0 0 0 0 0 0 6 0 0 % G
% His: 0 0 0 0 0 0 0 12 0 0 0 0 0 0 0 % H
% Ile: 0 6 0 0 6 0 0 0 6 0 6 0 0 0 18 % I
% Lys: 0 65 0 6 6 0 6 12 0 0 71 6 0 0 0 % K
% Leu: 6 0 0 0 0 0 0 0 0 83 0 0 0 0 36 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 % M
% Asn: 6 0 0 0 0 0 0 0 0 6 0 0 6 0 0 % N
% Pro: 0 0 77 83 12 12 0 0 0 0 6 0 0 0 0 % P
% Gln: 0 12 0 0 0 0 0 12 6 0 0 0 6 0 6 % Q
% Arg: 0 0 6 6 0 0 0 0 0 6 0 0 0 0 0 % R
% Ser: 6 0 0 6 0 0 6 59 24 0 0 0 0 0 18 % S
% Thr: 0 0 0 0 0 0 71 6 6 0 0 0 77 12 0 % T
% Val: 6 0 0 0 0 0 0 0 0 0 6 12 0 6 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 6 0 0 6 0 0 0 0 0 0 0 77 6 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _