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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PLK1 All Species: 35.76
Human Site: S326 Identified Species: 49.17
UniProt: P53350 Number Species: 16
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P53350 NP_005021.2 603 68255 S326 L T I P P R F S I A P S S L D
Chimpanzee Pan troglodytes XP_001163623 603 68223 S326 L T I P P R F S I A P S S L D
Rhesus Macaque Macaca mulatta XP_001092070 603 68240 S326 L T I P P R F S I A P S S L D
Dog Lupus familis XP_547091 832 93293 S554 L T I A P R F S I A P S S L D
Cat Felis silvestris
Mouse Mus musculus Q07832 603 68282 S326 L T I P P R F S I A P S S L D
Rat Rattus norvegicus Q62673 603 68295 S326 L T I P P R F S I A P S S L D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001505317 482 55166 K223 K P L T V L N K G L D S P V V
Chicken Gallus gallus NP_001025810 595 66904 S316 L T V A P R F S I A P S T L E
Frog Xenopus laevis P70032 598 68193 S317 L T V P P R F S I A P S T I D
Zebra Danio Brachydanio rerio NP_001003890 595 67743 S312 L T V P P R F S I A P S S L D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P52304 576 66955 G298 L T M A P R I G S N D T I E D
Honey Bee Apis mellifera XP_396707 575 66141 D296 L T M A P R L D M L E M H N Q
Nematode Worm Caenorhab. elegans P34331 649 73615 G311 L T M V P K F G G H E T S M M
Sea Urchin Strong. purpuratus XP_784826 587 66895 Q300 L T M A P R F Q V P V S S G R
Poplar Tree Populus trichocarpa
Maize Zea mays P0C8M8 626 70215 P309 P A S T L A C P P S T S Y L N
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9STV4 445 50410 G186 L S H K G Y N G A V A D I W S
Baker's Yeast Sacchar. cerevisiae P32562 705 81013 P354 S T V M S E A P N F E D I P E
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.8 99.8 69.5 N.A. 95 94.1 N.A. 69.3 80 79.7 74.3 N.A. 50.7 51.4 49.9 59.3
Protein Similarity: 100 99.8 99.8 71 N.A. 97.6 96.8 N.A. 74.7 89.8 90.5 87.5 N.A. 69.4 69.9 66.2 76.4
P-Site Identity: 100 100 100 93.3 N.A. 100 100 N.A. 6.6 73.3 80 93.3 N.A. 33.3 26.6 33.3 46.6
P-Site Similarity: 100 100 100 93.3 N.A. 100 100 N.A. 20 93.3 100 100 N.A. 46.6 40 60 60
Percent
Protein Identity: N.A. 31.7 N.A. 26 33.9 N.A.
Protein Similarity: N.A. 50.1 N.A. 40.4 51.6 N.A.
P-Site Identity: N.A. 13.3 N.A. 6.6 6.6 N.A.
P-Site Similarity: N.A. 26.6 N.A. 13.3 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 6 0 30 0 6 6 0 6 53 6 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 6 0 0 12 12 0 0 53 % D
% Glu: 0 0 0 0 0 6 0 0 0 0 18 0 0 6 12 % E
% Phe: 0 0 0 0 0 0 65 0 0 6 0 0 0 0 0 % F
% Gly: 0 0 0 0 6 0 0 18 12 0 0 0 0 6 0 % G
% His: 0 0 6 0 0 0 0 0 0 6 0 0 6 0 0 % H
% Ile: 0 0 36 0 0 0 6 0 53 0 0 0 18 6 0 % I
% Lys: 6 0 0 6 0 6 0 6 0 0 0 0 0 0 0 % K
% Leu: 83 0 6 0 6 6 6 0 0 12 0 0 0 53 0 % L
% Met: 0 0 24 6 0 0 0 0 6 0 0 6 0 6 6 % M
% Asn: 0 0 0 0 0 0 12 0 6 6 0 0 0 6 6 % N
% Pro: 6 6 0 42 77 0 0 12 6 6 53 0 6 6 0 % P
% Gln: 0 0 0 0 0 0 0 6 0 0 0 0 0 0 6 % Q
% Arg: 0 0 0 0 0 71 0 0 0 0 0 0 0 0 6 % R
% Ser: 6 6 6 0 6 0 0 53 6 6 0 71 53 0 6 % S
% Thr: 0 83 0 12 0 0 0 0 0 0 6 12 12 0 0 % T
% Val: 0 0 24 6 6 0 0 0 6 6 6 0 0 6 6 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 % W
% Tyr: 0 0 0 0 0 6 0 0 0 0 0 0 6 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _