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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MLF1 All Species: 10
Human Site: T216 Identified Species: 22
UniProt: P58340 Number Species: 10
    Phosphosite Substitution
    Charge Score: 0.1
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P58340 NP_001123629.1 268 30627 T216 G R H N L G N T R M R S V G H
Chimpanzee Pan troglodytes XP_516842 268 30670 T216 G R H N L E N T R M R G V G H
Rhesus Macaque Macaca mulatta XP_001102054 267 30586 T215 G R H N L E N T R M R S V G H
Dog Lupus familis XP_534319 303 34306 P251 Q W R N V E N P R M R S V G H
Cat Felis silvestris
Mouse Mus musculus Q9QWV4 267 30413 G216 I G R S G N T G M R S V G H E
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001508090 246 27769 H195 N N L V T P K H K S V H H G S
Chicken Gallus gallus XP_422822 275 31455 D223 S R S R C S L D S P K Y S S I
Frog Xenopus laevis NP_001088844 245 27988 A194 E F L N L D E A E G P S F D D
Zebra Danio Brachydanio rerio NP_001020687 250 28397 P199 E V G R Y R P P T A R S L D Y
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9NKV0 376 41359 G213 Q S R H H A G G M Q A I M P A
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_796343 284 31913 S216 G G R S V E E S R P P V L A V
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.5 93.6 67 N.A. 79.8 N.A. N.A. 60.4 55.2 49.6 38 N.A. 25.7 N.A. N.A. 35.5
Protein Similarity: 100 99.2 96.6 76.2 N.A. 90.3 N.A. N.A. 73.8 71.6 66 53.3 N.A. 38 N.A. N.A. 54.2
P-Site Identity: 100 86.6 93.3 60 N.A. 0 N.A. N.A. 6.6 6.6 20 13.3 N.A. 0 N.A. N.A. 13.3
P-Site Similarity: 100 86.6 93.3 66.6 N.A. 6.6 N.A. N.A. 13.3 13.3 20 26.6 N.A. 13.3 N.A. N.A. 40
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 10 0 10 0 10 10 0 0 10 10 % A
% Cys: 0 0 0 0 10 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 10 0 10 0 0 0 0 0 19 10 % D
% Glu: 19 0 0 0 0 37 19 0 10 0 0 0 0 0 10 % E
% Phe: 0 10 0 0 0 0 0 0 0 0 0 0 10 0 0 % F
% Gly: 37 19 10 0 10 10 10 19 0 10 0 10 10 46 0 % G
% His: 0 0 28 10 10 0 0 10 0 0 0 10 10 10 37 % H
% Ile: 10 0 0 0 0 0 0 0 0 0 0 10 0 0 10 % I
% Lys: 0 0 0 0 0 0 10 0 10 0 10 0 0 0 0 % K
% Leu: 0 0 19 0 37 0 10 0 0 0 0 0 19 0 0 % L
% Met: 0 0 0 0 0 0 0 0 19 37 0 0 10 0 0 % M
% Asn: 10 10 0 46 0 10 37 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 10 10 19 0 19 19 0 0 10 0 % P
% Gln: 19 0 0 0 0 0 0 0 0 10 0 0 0 0 0 % Q
% Arg: 0 37 37 19 0 10 0 0 46 10 46 0 0 0 0 % R
% Ser: 10 10 10 19 0 10 0 10 10 10 10 46 10 10 10 % S
% Thr: 0 0 0 0 10 0 10 28 10 0 0 0 0 0 0 % T
% Val: 0 10 0 10 19 0 0 0 0 0 10 19 37 0 10 % V
% Trp: 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 10 0 0 0 0 0 0 10 0 0 10 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _