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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: EIF4A1 All Species: 62.42
Human Site: T262 Identified Species: 85.83
UniProt: P60842 Number Species: 16
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P60842 NP_001407.1 406 46154 T262 R E E W K L D T L C D L Y E T
Chimpanzee Pan troglodytes A5A6N4 406 46165 T262 R E E W K L D T L C D L Y E T
Rhesus Macaque Macaca mulatta XP_001085678 406 46086 T262 R E E W K L D T L C D L Y E T
Dog Lupus familis XP_851852 406 46122 T262 R E E W K L D T L C D L Y E T
Cat Felis silvestris
Mouse Mus musculus P10630 407 46384 T263 R E E W K L D T L C D L Y E T
Rat Rattus norvegicus NP_001008336 407 46384 T263 R E E W K L D T L C D L Y E T
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511956 373 42398 F245 T I T Q A V I F I N T R R K V
Chicken Gallus gallus Q8JFP1 407 46353 T263 R E E W K L D T L C D L Y E T
Frog Xenopus laevis NP_001086413 406 46058 T262 R E E W K L D T L C D L Y E T
Zebra Danio Brachydanio rerio NP_938180 406 46235 T262 K E E W K L D T L C D L Y E T
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q02748 403 45860 T259 Q E N W K L G T L C D L Y D T
Honey Bee Apis mellifera XP_623285 423 48137 T279 R E E W K F E T L C D L Y D T
Nematode Worm Caenorhab. elegans P27639 402 45389 C258 K D E W K F D C L C D L Y N V
Sea Urchin Strong. purpuratus NP_001091916 426 48260 T282 K E E W K L D T L C D L Y E T
Poplar Tree Populus trichocarpa
Maize Zea mays Q41741 410 46518 T266 K E D W K L D T L C D L Y E T
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P41376 412 46686 T268 K E E W K L E T L C D L Y E T
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7RV88 397 44826 T253 K E E W K L D T L S D L Y E T
Conservation
Percent
Protein Identity: 100 99.7 99.2 99.5 N.A. 89.6 89.6 N.A. 83.9 90.6 95.3 89.6 N.A. 72.4 76.5 68.9 74.6
Protein Similarity: 100 99.7 99.7 99.7 N.A. 96.3 96.3 N.A. 86.2 96.3 99 96.8 N.A. 86.6 87.7 82.7 83.1
P-Site Identity: 100 100 100 100 N.A. 100 100 N.A. 0 100 100 93.3 N.A. 73.3 80 60 93.3
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 20 100 100 100 N.A. 86.6 93.3 73.3 100
Percent
Protein Identity: N.A. 68 N.A. 68.2 N.A. 75.8
Protein Similarity: N.A. 81.4 N.A. 81 N.A. 87.1
P-Site Identity: N.A. 86.6 N.A. 86.6 N.A. 86.6
P-Site Similarity: N.A. 100 N.A. 100 N.A. 93.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 6 0 89 0 0 0 0 0 % C
% Asp: 0 6 6 0 0 0 77 0 0 0 95 0 0 12 0 % D
% Glu: 0 89 83 0 0 0 12 0 0 0 0 0 0 77 0 % E
% Phe: 0 0 0 0 0 12 0 6 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 6 0 0 0 0 6 0 6 0 0 0 0 0 0 % I
% Lys: 36 0 0 0 95 0 0 0 0 0 0 0 0 6 0 % K
% Leu: 0 0 0 0 0 83 0 0 95 0 0 95 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 6 0 0 0 0 0 0 6 0 0 0 6 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 6 0 0 6 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 53 0 0 0 0 0 0 0 0 0 0 6 6 0 0 % R
% Ser: 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 % S
% Thr: 6 0 6 0 0 0 0 89 0 0 6 0 0 0 89 % T
% Val: 0 0 0 0 0 6 0 0 0 0 0 0 0 0 12 % V
% Trp: 0 0 0 95 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 95 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _