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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: EIF4A1 All Species: 61.82
Human Site: T298 Identified Species: 85
UniProt: P60842 Number Species: 16
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P60842 NP_001407.1 406 46154 T298 K M H A R D F T V S A M H G D
Chimpanzee Pan troglodytes A5A6N4 406 46165 T298 K M H A R D F T V S A M H G D
Rhesus Macaque Macaca mulatta XP_001085678 406 46086 T298 K M H A R D F T V S A M H G D
Dog Lupus familis XP_851852 406 46122 T298 K M H A R D F T V S A M H G D
Cat Felis silvestris
Mouse Mus musculus P10630 407 46384 T299 K M H A R D F T V S A L H G D
Rat Rattus norvegicus NP_001008336 407 46384 T299 K M H A R D F T V S A L H G D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511956 373 42398 I281 D Q K E R D V I M R E F R S G
Chicken Gallus gallus Q8JFP1 407 46353 T299 K M H A R D F T V S A L H G D
Frog Xenopus laevis NP_001086413 406 46058 T298 K M H A R D F T V S A L H G D
Zebra Danio Brachydanio rerio NP_938180 406 46235 T298 K M H A R D F T V S A L H G D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q02748 403 45860 T295 E M S I H N F T V S A M H G D
Honey Bee Apis mellifera XP_623285 423 48137 T315 S M R T R D F T V S A M H G D
Nematode Worm Caenorhab. elegans P27639 402 45389 T294 K M T E N Q F T V S C L H G D
Sea Urchin Strong. purpuratus NP_001091916 426 48260 T318 K M H E R D F T V S I M H R D
Poplar Tree Populus trichocarpa
Maize Zea mays Q41741 410 46518 T302 K M R S R D H T V S A T H G D
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P41376 412 46686 T304 K M R S R D H T V S A T H G D
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7RV88 397 44826 T289 K L T A R D F T V S A M H G D
Conservation
Percent
Protein Identity: 100 99.7 99.2 99.5 N.A. 89.6 89.6 N.A. 83.9 90.6 95.3 89.6 N.A. 72.4 76.5 68.9 74.6
Protein Similarity: 100 99.7 99.7 99.7 N.A. 96.3 96.3 N.A. 86.2 96.3 99 96.8 N.A. 86.6 87.7 82.7 83.1
P-Site Identity: 100 100 100 100 N.A. 93.3 93.3 N.A. 13.3 93.3 93.3 93.3 N.A. 66.6 80 60 80
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 20 100 100 100 N.A. 80 80 66.6 80
Percent
Protein Identity: N.A. 68 N.A. 68.2 N.A. 75.8
Protein Similarity: N.A. 81.4 N.A. 81 N.A. 87.1
P-Site Identity: N.A. 73.3 N.A. 73.3 N.A. 86.6
P-Site Similarity: N.A. 80 N.A. 80 N.A. 93.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 59 0 0 0 0 0 0 83 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 % C
% Asp: 6 0 0 0 0 89 0 0 0 0 0 0 0 0 95 % D
% Glu: 6 0 0 18 0 0 0 0 0 0 6 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 83 0 0 0 0 6 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 89 6 % G
% His: 0 0 59 0 6 0 12 0 0 0 0 0 95 0 0 % H
% Ile: 0 0 0 6 0 0 0 6 0 0 6 0 0 0 0 % I
% Lys: 83 0 6 0 0 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 0 6 0 0 0 0 0 0 0 0 0 36 0 0 0 % L
% Met: 0 89 0 0 0 0 0 0 6 0 0 48 0 0 0 % M
% Asn: 0 0 0 0 6 6 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 6 0 0 0 6 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 18 0 89 0 0 0 0 6 0 0 6 6 0 % R
% Ser: 6 0 6 12 0 0 0 0 0 95 0 0 0 6 0 % S
% Thr: 0 0 12 6 0 0 0 95 0 0 0 12 0 0 0 % T
% Val: 0 0 0 0 0 0 6 0 95 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _