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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: EIF4A1 All Species: 42.73
Human Site: Y170 Identified Species: 58.75
UniProt: P60842 Number Species: 16
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P60842 NP_001407.1 406 46154 Y170 F D M L N R R Y L S P K Y I K
Chimpanzee Pan troglodytes A5A6N4 406 46165 Y170 F D M L N R R Y L S P K Y I K
Rhesus Macaque Macaca mulatta XP_001085678 406 46086 Y170 F D M L N R R Y L S P K Y I K
Dog Lupus familis XP_851852 406 46122 Y170 F D M L N R R Y L S P K Y I K
Cat Felis silvestris
Mouse Mus musculus P10630 407 46384 Y171 F D M L N R R Y L S P K W I K
Rat Rattus norvegicus NP_001008336 407 46384 Y171 F D M L N R R Y L S P K W I K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511956 373 42398 S156 D E A D E M L S R G F K D Q I
Chicken Gallus gallus Q8JFP1 407 46353 Y171 F D M L N R R Y L S P K W I K
Frog Xenopus laevis NP_001086413 406 46058 Y170 F D M L N R R Y L S P K Y I K
Zebra Danio Brachydanio rerio NP_938180 406 46235 Y170 F D M L N R R Y L S P K Y I K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q02748 403 45860 V167 Y D M I N R K V L R T Q Y I K
Honey Bee Apis mellifera XP_623285 423 48137 A187 Y D M I S R R A L R A S S I K
Nematode Worm Caenorhab. elegans P27639 402 45389 A166 G D M I N R N A L D T S R I K
Sea Urchin Strong. purpuratus NP_001091916 426 48260 V190 F D M L K R K V L S P F K I C
Poplar Tree Populus trichocarpa
Maize Zea mays Q41741 410 46518 S174 F D M L R R Q S L R P D N I K
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P41376 412 46686 S176 F D M L K R Q S L R A D N I K
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7RV88 397 44826 L162 D M I Q R R F L K T D S M K M
Conservation
Percent
Protein Identity: 100 99.7 99.2 99.5 N.A. 89.6 89.6 N.A. 83.9 90.6 95.3 89.6 N.A. 72.4 76.5 68.9 74.6
Protein Similarity: 100 99.7 99.7 99.7 N.A. 96.3 96.3 N.A. 86.2 96.3 99 96.8 N.A. 86.6 87.7 82.7 83.1
P-Site Identity: 100 100 100 100 N.A. 93.3 93.3 N.A. 6.6 93.3 100 100 N.A. 53.3 46.6 46.6 60
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 13.3 100 100 100 N.A. 80 66.6 53.3 66.6
Percent
Protein Identity: N.A. 68 N.A. 68.2 N.A. 75.8
Protein Similarity: N.A. 81.4 N.A. 81 N.A. 87.1
P-Site Identity: N.A. 60 N.A. 53.3 N.A. 6.6
P-Site Similarity: N.A. 66.6 N.A. 60 N.A. 20
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 6 0 0 0 0 12 0 0 12 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 % C
% Asp: 12 89 0 6 0 0 0 0 0 6 6 12 6 0 0 % D
% Glu: 0 6 0 0 6 0 0 0 0 0 0 0 0 0 0 % E
% Phe: 71 0 0 0 0 0 6 0 0 0 6 6 0 0 0 % F
% Gly: 6 0 0 0 0 0 0 0 0 6 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 6 18 0 0 0 0 0 0 0 0 0 89 6 % I
% Lys: 0 0 0 0 12 0 12 0 6 0 0 59 6 6 83 % K
% Leu: 0 0 0 71 0 0 6 6 89 0 0 0 0 0 0 % L
% Met: 0 6 89 0 0 6 0 0 0 0 0 0 6 0 6 % M
% Asn: 0 0 0 0 65 0 6 0 0 0 0 0 12 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 65 0 0 0 0 % P
% Gln: 0 0 0 6 0 0 12 0 0 0 0 6 0 6 0 % Q
% Arg: 0 0 0 0 12 95 59 0 6 24 0 0 6 0 0 % R
% Ser: 0 0 0 0 6 0 0 18 0 59 0 18 6 0 0 % S
% Thr: 0 0 0 0 0 0 0 0 0 6 12 0 0 0 0 % T
% Val: 0 0 0 0 0 0 0 12 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 18 0 0 % W
% Tyr: 12 0 0 0 0 0 0 53 0 0 0 0 42 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _