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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: SMAD3 All Species: 16.67
Human Site: S192 Identified Species: 30.56
UniProt: P84022 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.42
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P84022 NP_001138574.1 425 48081 S192 L S E D G E T S D H Q M N H S
Chimpanzee Pan troglodytes XP_001159972 710 77879 S477 L S E D G E T S D H Q M N H S
Rhesus Macaque Macaca mulatta XP_001111262 425 48034 S192 L S E D G E T S D H Q M N H S
Dog Lupus familis XP_866258 457 51167 T224 Y I S E D G E T S D Q Q L N Q
Cat Felis silvestris
Mouse Mus musculus Q62432 467 52248 D234 S E D G E T S D Q Q L N Q S M
Rat Rattus norvegicus O70436 467 52221 D234 S E D G E T S D Q Q L N Q S M
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001505221 437 48895 D204 S E D G E T S D Q Q L N Q S M
Chicken Gallus gallus P84023 426 48233 S193 L S E D G E T S D H Q M N P S
Frog Xenopus laevis NP_001079320 425 48022 T192 Y L S E D G E T S D Q M N H S
Zebra Danio Brachydanio rerio Q9I9P9 468 52435 D235 S E D G E A S D Q Q M N Q S M
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P42003 455 50486 P222 D S L A G T P P P A Y S P S E
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q02330 418 47907 F183 V N F T A N Q F H Q Y N P N G
Sea Urchin Strong. purpuratus NP_001075435 427 48320 D194 P P P G Y V S D D G E T S E Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 59.8 99.7 85.3 N.A. 83.9 83.7 N.A. 89.9 98.8 95.7 83.3 N.A. 60 N.A. 49.6 78.6
Protein Similarity: 100 59.8 99.7 89.5 N.A. 88 88 N.A. 94.2 99.5 98.8 87.1 N.A. 73.1 N.A. 64.2 88.7
P-Site Identity: 100 100 100 6.6 N.A. 0 0 N.A. 0 93.3 33.3 0 N.A. 13.3 N.A. 0 6.6
P-Site Similarity: 100 100 100 26.6 N.A. 13.3 13.3 N.A. 13.3 93.3 46.6 13.3 N.A. 13.3 N.A. 20 26.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 8 8 8 0 0 0 8 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 8 0 31 31 16 0 0 39 39 16 0 0 0 0 0 % D
% Glu: 0 31 31 16 31 31 16 0 0 0 8 0 0 8 8 % E
% Phe: 0 0 8 0 0 0 0 8 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 39 39 16 0 0 0 8 0 0 0 0 8 % G
% His: 0 0 0 0 0 0 0 0 8 31 0 0 0 31 0 % H
% Ile: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % I
% Lys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 31 8 8 0 0 0 0 0 0 0 24 0 8 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 8 39 0 0 31 % M
% Asn: 0 8 0 0 0 8 0 0 0 0 0 39 39 16 0 % N
% Pro: 8 8 8 0 0 0 8 8 8 0 0 0 16 8 0 % P
% Gln: 0 0 0 0 0 0 8 0 31 39 47 8 31 0 16 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 31 39 16 0 0 0 39 31 16 0 0 8 8 39 39 % S
% Thr: 0 0 0 8 0 31 31 16 0 0 0 8 0 0 0 % T
% Val: 8 0 0 0 0 8 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 16 0 0 0 8 0 0 0 0 0 16 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _