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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: SMAD3 All Species: 16.97
Human Site: S199 Identified Species: 31.11
UniProt: P84022 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P84022 NP_001138574.1 425 48081 S199 S D H Q M N H S M D A G S P N
Chimpanzee Pan troglodytes XP_001159972 710 77879 S484 S D H Q M N H S M D A G S P N
Rhesus Macaque Macaca mulatta XP_001111262 425 48034 S199 S D H Q M N H S M D A G S P N
Dog Lupus familis XP_866258 457 51167 Q231 T S D Q Q L N Q S M D T G S P
Cat Felis silvestris
Mouse Mus musculus Q62432 467 52248 M241 D Q Q L N Q S M D T G S P A E
Rat Rattus norvegicus O70436 467 52221 M241 D Q Q L N Q S M D T G S P A E
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001505221 437 48895 M211 D Q Q L N Q S M D T G S P A E
Chicken Gallus gallus P84023 426 48233 S200 S D H Q M N P S M D A G S P N
Frog Xenopus laevis NP_001079320 425 48022 S199 T S D Q M N H S I D T G S P N
Zebra Danio Brachydanio rerio Q9I9P9 468 52435 M242 D Q Q M N Q S M D T G S P A E
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P42003 455 50486 E229 P P A Y S P S E D G N S N N P
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q02330 418 47907 G190 F H Q Y N P N G I E E M D T S
Sea Urchin Strong. purpuratus NP_001075435 427 48320 Q201 D D G E T S E Q N M D T S S T
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 59.8 99.7 85.3 N.A. 83.9 83.7 N.A. 89.9 98.8 95.7 83.3 N.A. 60 N.A. 49.6 78.6
Protein Similarity: 100 59.8 99.7 89.5 N.A. 88 88 N.A. 94.2 99.5 98.8 87.1 N.A. 73.1 N.A. 64.2 88.7
P-Site Identity: 100 100 100 6.6 N.A. 0 0 N.A. 0 93.3 66.6 0 N.A. 0 N.A. 0 13.3
P-Site Similarity: 100 100 100 20 N.A. 0 0 N.A. 0 93.3 80 0 N.A. 6.6 N.A. 26.6 26.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 8 0 0 0 0 0 0 0 31 0 0 31 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 39 39 16 0 0 0 0 0 39 39 16 0 8 0 0 % D
% Glu: 0 0 0 8 0 0 8 8 0 8 8 0 0 0 31 % E
% Phe: 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 8 0 0 0 0 8 0 8 31 39 8 0 0 % G
% His: 0 8 31 0 0 0 31 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 16 0 0 0 0 0 0 % I
% Lys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 0 0 0 24 0 8 0 0 0 0 0 0 0 0 0 % L
% Met: 0 0 0 8 39 0 0 31 31 16 0 8 0 0 0 % M
% Asn: 0 0 0 0 39 39 16 0 8 0 8 0 8 8 39 % N
% Pro: 8 8 0 0 0 16 8 0 0 0 0 0 31 39 16 % P
% Gln: 0 31 39 47 8 31 0 16 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 31 16 0 0 8 8 39 39 8 0 0 39 47 16 8 % S
% Thr: 16 0 0 0 8 0 0 0 0 31 8 16 0 8 8 % T
% Val: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 16 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _