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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: SMAD3 All Species: 33.33
Human Site: S213 Identified Species: 61.11
UniProt: P84022 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P84022 NP_001138574.1 425 48081 S213 N L S P N P M S P A H N N L D
Chimpanzee Pan troglodytes XP_001159972 710 77879 S498 N L S P N P M S P A H N N L D
Rhesus Macaque Macaca mulatta XP_001111262 425 48034 S213 N L S P N P M S P A H N N L D
Dog Lupus familis XP_866258 457 51167 T245 P A E L S P T T L S P V N H N
Cat Felis silvestris
Mouse Mus musculus Q62432 467 52248 S255 E L S P T T L S P V N H S L D
Rat Rattus norvegicus O70436 467 52221 S255 E L S P T T L S P V N H S L D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001505221 437 48895 S225 E L S P S T L S P V N H S L D
Chicken Gallus gallus P84023 426 48233 S214 N L S P N P M S P A H N N L D
Frog Xenopus laevis NP_001079320 425 48022 S213 N L S P N S M S P A H S N M D
Zebra Danio Brachydanio rerio Q9I9P9 468 52435 S256 E L S P S T L S P V N H G M D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P42003 455 50486 L243 P N D G G Q L L D A Q M G D V
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q02330 418 47907 P204 S Q K F D I P P G V P T C L V
Sea Urchin Strong. purpuratus NP_001075435 427 48320 P215 T N G S S T A P Q S S E S L D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 59.8 99.7 85.3 N.A. 83.9 83.7 N.A. 89.9 98.8 95.7 83.3 N.A. 60 N.A. 49.6 78.6
Protein Similarity: 100 59.8 99.7 89.5 N.A. 88 88 N.A. 94.2 99.5 98.8 87.1 N.A. 73.1 N.A. 64.2 88.7
P-Site Identity: 100 100 100 13.3 N.A. 46.6 46.6 N.A. 46.6 100 80 40 N.A. 6.6 N.A. 6.6 13.3
P-Site Similarity: 100 100 100 40 N.A. 73.3 73.3 N.A. 80 100 93.3 73.3 N.A. 13.3 N.A. 20 33.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 0 0 0 0 8 0 0 47 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 % C
% Asp: 0 0 8 0 8 0 0 0 8 0 0 0 0 8 77 % D
% Glu: 31 0 8 0 0 0 0 0 0 0 0 8 0 0 0 % E
% Phe: 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 8 8 8 0 0 0 8 0 0 0 16 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 39 31 0 8 0 % H
% Ile: 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 % I
% Lys: 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 0 70 0 8 0 0 39 8 8 0 0 0 0 70 0 % L
% Met: 0 0 0 0 0 0 39 0 0 0 0 8 0 16 0 % M
% Asn: 39 16 0 0 39 0 0 0 0 0 31 31 47 0 8 % N
% Pro: 16 0 0 70 0 39 8 16 70 0 16 0 0 0 0 % P
% Gln: 0 8 0 0 0 8 0 0 8 0 8 0 0 0 0 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 8 0 70 8 31 8 0 70 0 16 8 8 31 0 0 % S
% Thr: 8 0 0 0 16 39 8 8 0 0 0 8 0 0 0 % T
% Val: 0 0 0 0 0 0 0 0 0 39 0 8 0 0 16 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _