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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: SMAD3 All Species: 13.64
Human Site: T132 Identified Species: 25
UniProt: P84022 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P84022 NP_001138574.1 425 48081 T132 Y H Y Q R V E T P V L P P V L
Chimpanzee Pan troglodytes XP_001159972 710 77879 T417 Y H Y Q R V E T P V L P P V L
Rhesus Macaque Macaca mulatta XP_001111262 425 48034 T132 Y H Y Q R V E T P V L P P V L
Dog Lupus familis XP_866258 457 51167 D164 L T E L P P L D D Y T H S I P
Cat Felis silvestris
Mouse Mus musculus Q62432 467 52248 V174 Y Q R V E T P V L P P V L V P
Rat Rattus norvegicus O70436 467 52221 V174 Y Q R V E T P V L P P V L V P
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001505221 437 48895 V144 Y Q R V E T P V L P P V L V P
Chicken Gallus gallus P84023 426 48233 P133 H Y Q R V E T P V L P P V L V
Frog Xenopus laevis NP_001079320 425 48022 T132 Y H Y Q R V E T P V L P P V L
Zebra Danio Brachydanio rerio Q9I9P9 468 52435 V175 Y Q R V E T P V L P P V L V P
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P42003 455 50486 P162 P R H S E F A P G H S M L Q F
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q02330 418 47907 Y123 K D I C I N P Y H Y K R V H A
Sea Urchin Strong. purpuratus NP_001075435 427 48320 P134 R V E T P V L P P V L V P R I
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 59.8 99.7 85.3 N.A. 83.9 83.7 N.A. 89.9 98.8 95.7 83.3 N.A. 60 N.A. 49.6 78.6
Protein Similarity: 100 59.8 99.7 89.5 N.A. 88 88 N.A. 94.2 99.5 98.8 87.1 N.A. 73.1 N.A. 64.2 88.7
P-Site Identity: 100 100 100 0 N.A. 13.3 13.3 N.A. 13.3 6.6 100 13.3 N.A. 0 N.A. 0 33.3
P-Site Similarity: 100 100 100 6.6 N.A. 13.3 13.3 N.A. 13.3 46.6 100 13.3 N.A. 6.6 N.A. 0 40
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 8 0 0 0 0 0 0 0 8 % A
% Cys: 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 8 0 0 0 0 0 8 8 0 0 0 0 0 0 % D
% Glu: 0 0 16 0 39 8 31 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 8 0 0 0 0 0 0 0 0 8 % F
% Gly: 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 % G
% His: 8 31 8 0 0 0 0 0 8 8 0 8 0 8 0 % H
% Ile: 0 0 8 0 8 0 0 0 0 0 0 0 0 8 8 % I
% Lys: 8 0 0 0 0 0 0 0 0 0 8 0 0 0 0 % K
% Leu: 8 0 0 8 0 0 16 0 31 8 39 0 39 8 31 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 % M
% Asn: 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 % N
% Pro: 8 0 0 0 16 8 39 24 39 31 39 39 39 0 39 % P
% Gln: 0 31 8 31 0 0 0 0 0 0 0 0 0 8 0 % Q
% Arg: 8 8 31 8 31 0 0 0 0 0 0 8 0 8 0 % R
% Ser: 0 0 0 8 0 0 0 0 0 0 8 0 8 0 0 % S
% Thr: 0 8 0 8 0 31 8 31 0 0 8 0 0 0 0 % T
% Val: 0 8 0 31 8 39 0 31 8 39 0 39 16 62 8 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 62 8 31 0 0 0 0 8 0 16 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _