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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: SLC25A3 All Species: 33.94
Human Site: S255 Identified Species: 49.78
UniProt: Q00325 Number Species: 15
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q00325 NP_002626.1 362 40095 S255 F V V P K P R S E C S K P E Q
Chimpanzee Pan troglodytes XP_509289 315 34683 G218 G L K A F Y K G V A P L W M R
Rhesus Macaque Macaca mulatta XP_001083690 362 40156 S255 F V V P K P R S E C S K P E Q
Dog Lupus familis XP_866402 362 39917 S255 F V V P K P R S E C S K A E Q
Cat Felis silvestris
Mouse Mus musculus Q8VEM8 357 39614 S250 F V V P K P R S E C T K A E Q
Rat Rattus norvegicus P16036 356 39427 S249 F V V P K P R S E C T K A E Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001506076 369 41102 S262 Y V V P K P R S E C S K A E Q
Chicken Gallus gallus NP_001006236 335 37403 V238 S K G E Q L V V T F I A G Y I
Frog Xenopus laevis NP_001080195 359 39888 S252 Y V V P K P R S E C S K S E Q
Zebra Danio Brachydanio rerio NP_998887 356 39288 N249 Y V V P K P R N E C S K A E Q
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_611468 374 40535 A265 Y V V P K P R A D C T K G E Q
Honey Bee Apis mellifera XP_396960 354 39111 Q248 Y V V P K P R Q E C S K G E Q
Nematode Worm Caenorhab. elegans P40614 340 36655 V242 C S K A E Q L V V T F V A G Y
Sea Urchin Strong. purpuratus XP_783048 356 39100 S252 H V V P K P R S D C S K G E Q
Poplar Tree Populus trichocarpa
Maize Zea mays NP_001140930 356 39513 S249 F V V P K P R S E C T K A E Q
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P40035 300 33510 E203 S V L P K K K E E M N A L Q Q
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 83.4 98.3 95.3 N.A. 88.6 88.1 N.A. 81.3 79 82.8 79.5 N.A. 67.3 67.1 65.4 69.3
Protein Similarity: 100 85.9 99.4 96.1 N.A. 93.9 93.6 N.A. 87.2 84.2 90 87 N.A. 79.9 79.5 75.6 81.2
P-Site Identity: 100 0 100 93.3 N.A. 86.6 86.6 N.A. 86.6 0 86.6 80 N.A. 66.6 80 0 80
P-Site Similarity: 100 20 100 93.3 N.A. 93.3 93.3 N.A. 93.3 6.6 93.3 93.3 N.A. 93.3 86.6 6.6 86.6
Percent
Protein Identity: N.A. 88.6 N.A. N.A. 41.9 N.A.
Protein Similarity: N.A. 93.9 N.A. N.A. 56.9 N.A.
P-Site Identity: N.A. 86.6 N.A. N.A. 33.3 N.A.
P-Site Similarity: N.A. 93.3 N.A. N.A. 60 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 13 0 0 0 7 0 7 0 13 44 0 0 % A
% Cys: 7 0 0 0 0 0 0 0 0 75 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 13 0 0 0 0 0 0 % D
% Glu: 0 0 0 7 7 0 0 7 69 0 0 0 0 75 0 % E
% Phe: 38 0 0 0 7 0 0 0 0 7 7 0 0 0 0 % F
% Gly: 7 0 7 0 0 0 0 7 0 0 0 0 25 7 0 % G
% His: 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 7 0 0 0 7 % I
% Lys: 0 7 13 0 82 7 13 0 0 0 0 75 0 0 0 % K
% Leu: 0 7 7 0 0 7 7 0 0 0 0 7 7 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 7 0 0 0 7 0 % M
% Asn: 0 0 0 0 0 0 0 7 0 0 7 0 0 0 0 % N
% Pro: 0 0 0 82 0 75 0 0 0 0 7 0 13 0 0 % P
% Gln: 0 0 0 0 7 7 0 7 0 0 0 0 0 7 82 % Q
% Arg: 0 0 0 0 0 0 75 0 0 0 0 0 0 0 7 % R
% Ser: 13 7 0 0 0 0 0 57 0 0 50 0 7 0 0 % S
% Thr: 0 0 0 0 0 0 0 0 7 7 25 0 0 0 0 % T
% Val: 0 82 75 0 0 0 7 13 13 0 0 7 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 % W
% Tyr: 32 0 0 0 0 7 0 0 0 0 0 0 0 7 7 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _