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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKP All Species: 15.76
Human Site: T294 Identified Species: 28.89
UniProt: Q01813 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q01813 NP_002618.1 784 85596 T294 K I K E L V V T Q L G Y D T R
Chimpanzee Pan troglodytes XP_507625 746 81795 P276 H V Q R G G T P S A F D R I L
Rhesus Macaque Macaca mulatta XP_001118490 717 78539 D272 A L L E A T P D T P A C V V S
Dog Lupus familis XP_535201 901 98160 T405 Q I K E L V V T Q L G Y D T R
Cat Felis silvestris
Mouse Mus musculus Q9WUA3 784 85437 K293 K I K E L V V K N L G F D T R
Rat Rattus norvegicus P47860 788 85702 T294 K I K E L V V T N L G F D T R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511162 864 94802 K365 K V K D L V V K Q L G F D T R
Chicken Gallus gallus NP_989554 769 83907 I283 G Y D T R V T I L G H V Q R G
Frog Xenopus laevis NP_001091319 786 86623 K286 L V K D L V M K R L G F D T R
Zebra Danio Brachydanio rerio XP_002666643 787 86261 S287 H V K N L V V S R L G F D T R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P52034 788 86630 E287 D V K K V I D E R L K H D A R
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q27483 756 83282 T278 Q V K E E I E T R L Q L E V R
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P16862 959 104599 D468 D V H K V L V D R L G L D T R
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 94.7 89.4 78.9 N.A. 88.5 87.9 N.A. 77.1 70.7 81.3 79.6 N.A. 58.8 N.A. 40.6 N.A.
Protein Similarity: 100 95 90.4 83.5 N.A. 93.6 93.4 N.A. 84.1 82 90.5 89.3 N.A. 75.8 N.A. 59.6 N.A.
P-Site Identity: 100 0 6.6 93.3 N.A. 80 86.6 N.A. 73.3 6.6 53.3 60 N.A. 26.6 N.A. 33.3 N.A.
P-Site Similarity: 100 13.3 13.3 100 N.A. 86.6 93.3 N.A. 93.3 6.6 86.6 86.6 N.A. 66.6 N.A. 66.6 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 34.8 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 52 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 40 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 73.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 0 0 0 8 0 0 0 0 8 8 0 0 8 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 % C
% Asp: 16 0 8 16 0 0 8 16 0 0 0 8 70 0 0 % D
% Glu: 0 0 0 47 8 0 8 8 0 0 0 0 8 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 8 39 0 0 0 % F
% Gly: 8 0 0 0 8 8 0 0 0 8 62 0 0 0 8 % G
% His: 16 0 8 0 0 0 0 0 0 0 8 8 0 0 0 % H
% Ile: 0 31 0 0 0 16 0 8 0 0 0 0 0 8 0 % I
% Lys: 31 0 70 16 0 0 0 24 0 0 8 0 0 0 0 % K
% Leu: 8 8 8 0 54 8 0 0 8 77 0 16 0 0 8 % L
% Met: 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 8 0 0 0 0 16 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 8 8 0 8 0 0 0 0 0 % P
% Gln: 16 0 8 0 0 0 0 0 24 0 8 0 8 0 0 % Q
% Arg: 0 0 0 8 8 0 0 0 39 0 0 0 8 8 77 % R
% Ser: 0 0 0 0 0 0 0 8 8 0 0 0 0 0 8 % S
% Thr: 0 0 0 8 0 8 16 31 8 0 0 0 0 62 0 % T
% Val: 0 54 0 0 16 62 54 0 0 0 0 8 8 16 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 8 0 0 0 0 0 0 0 0 0 16 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _