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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2B All Species: 25.15
Human Site: S1321 Identified Species: 39.52
UniProt: Q02880 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q02880 NP_001059.2 1626 183267 S1321 V R K T P T S S G K P S A K K
Chimpanzee Pan troglodytes XP_516332 1634 184611 S1329 V R K T P T S S G K P S A K K
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 S1315 V R K T P T S S G K P S A K K
Dog Lupus familis XP_534241 2041 231068 S1739 V R K T P T S S G K S S A K K
Cat Felis silvestris
Mouse Mus musculus Q64511 1612 181890 T1309 V R K T P T S T G K T N A K K
Rat Rattus norvegicus P41516 1526 173202 V1241 R K I K S E N V E G T P A E D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001507216 1923 216719 S1624 V R K T P L S S T K S S A K K
Chicken Gallus gallus O42131 1627 183228 S1326 R V R R A P S S T K S S A K K
Frog Xenopus laevis NP_001082502 1579 178601 T1291 V K D K K Q A T L P F K P V K
Zebra Danio Brachydanio rerio NP_001038656 1618 182448 G1323 P R Q R K P A G A K G P A K K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 E1162 S L R K K T P E M L W L D D L
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 A1235 K Q E A R E K A D Q D A S I K
Sea Urchin Strong. purpuratus XP_783546 1448 163750 K1163 L W S L T K E K K N D L L K Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 Q1188 Q Q A I E A A Q K K I R A K S
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 L1143 K Q E K E T E L E N L L K L S
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.3 98.5 78.1 N.A. 95.6 66.2 N.A. 79.3 89.2 66.6 73.6 N.A. 50.3 N.A. 49.5 57.3
Protein Similarity: 100 98.5 99.1 78.8 N.A. 97.2 78.1 N.A. 81.8 93.8 78.7 83.8 N.A. 64.3 N.A. 65.8 69.9
P-Site Identity: 100 100 100 93.3 N.A. 80 6.6 N.A. 80 46.6 13.3 33.3 N.A. 6.6 N.A. 6.6 6.6
P-Site Similarity: 100 100 100 93.3 N.A. 93.3 26.6 N.A. 80 53.3 33.3 46.6 N.A. 13.3 N.A. 46.6 20
Percent
Protein Identity: N.A. N.A. N.A. 43.3 40.7 N.A.
Protein Similarity: N.A. N.A. N.A. 59 56.8 N.A.
P-Site Identity: N.A. N.A. N.A. 20 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 33.3 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 7 7 7 20 7 7 0 0 7 67 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 7 0 0 0 0 0 7 0 14 0 7 7 7 % D
% Glu: 0 0 14 0 14 14 14 7 14 0 0 0 0 7 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 7 34 7 7 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 7 7 0 0 0 0 0 0 7 0 0 7 0 % I
% Lys: 14 14 40 27 20 7 7 7 14 60 0 7 7 67 67 % K
% Leu: 7 7 0 7 0 7 0 7 7 7 7 20 7 7 7 % L
% Met: 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 7 0 0 14 0 7 0 0 0 % N
% Pro: 7 0 0 0 40 14 7 0 0 7 20 14 7 0 0 % P
% Gln: 7 20 7 0 0 7 0 7 0 7 0 0 0 0 7 % Q
% Arg: 14 47 14 14 7 0 0 0 0 0 0 7 0 0 0 % R
% Ser: 7 0 7 0 7 0 47 40 0 0 20 40 7 0 14 % S
% Thr: 0 0 0 40 7 47 0 14 14 0 14 0 0 0 0 % T
% Val: 47 7 0 0 0 0 0 7 0 0 0 0 0 7 0 % V
% Trp: 0 7 0 0 0 0 0 0 0 0 7 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _