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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2B All Species: 19.39
Human Site: S1473 Identified Species: 30.48
UniProt: Q02880 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q02880 NP_001059.2 1626 183267 S1473 S N E E D S A S V F S P S F G
Chimpanzee Pan troglodytes XP_516332 1634 184611 S1481 S N E E D S A S V F S P S F G
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 S1467 S N E E D S A S V F S P S F G
Dog Lupus familis XP_534241 2041 231068 S1890 S N E E D S A S V F S P S F G
Cat Felis silvestris
Mouse Mus musculus Q64511 1612 181890 S1460 S N E E D T A S V F A P S F G
Rat Rattus norvegicus P41516 1526 173202 S1383 L E S D G K D S V P A S P G A
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001507216 1923 216719 P1773 S Y E E D S A P V F S S S F A
Chicken Gallus gallus O42131 1627 183228 P1479 S D E E D S T P V F S S P F A
Frog Xenopus laevis NP_001082502 1579 178601 A1435 A P S A P E P A P R A K V S S
Zebra Danio Brachydanio rerio NP_001038656 1618 182448 D1465 D D M K S D S D G D R G P V F
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 K1304 G G S K T S P K A K K A V V K
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 G1377 S K P K P R T G K G A A K A E
Sea Urchin Strong. purpuratus XP_783546 1448 163750 P1305 S V A L S D K P A G A K P K T
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 A1330 A E T S K A I A V D D D D D D
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 E1285 K E D K D E G E L S K I S N K
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.3 98.5 78.1 N.A. 95.6 66.2 N.A. 79.3 89.2 66.6 73.6 N.A. 50.3 N.A. 49.5 57.3
Protein Similarity: 100 98.5 99.1 78.8 N.A. 97.2 78.1 N.A. 81.8 93.8 78.7 83.8 N.A. 64.3 N.A. 65.8 69.9
P-Site Identity: 100 100 100 100 N.A. 86.6 13.3 N.A. 73.3 60 0 0 N.A. 6.6 N.A. 6.6 6.6
P-Site Similarity: 100 100 100 100 N.A. 100 26.6 N.A. 73.3 66.6 20 20 N.A. 13.3 N.A. 20 13.3
Percent
Protein Identity: N.A. N.A. N.A. 43.3 40.7 N.A.
Protein Similarity: N.A. N.A. N.A. 59 56.8 N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 26.6 33.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 14 0 7 7 0 7 40 14 14 0 34 14 0 7 20 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 7 14 7 7 54 14 7 7 0 14 7 7 7 7 7 % D
% Glu: 0 20 47 47 0 14 0 7 0 0 0 0 0 0 7 % E
% Phe: 0 0 0 0 0 0 0 0 0 47 0 0 0 47 7 % F
% Gly: 7 7 0 0 7 0 7 7 7 14 0 7 0 7 34 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 7 0 0 0 0 7 0 0 0 % I
% Lys: 7 7 0 27 7 7 7 7 7 7 14 14 7 7 14 % K
% Leu: 7 0 0 7 0 0 0 0 7 0 0 0 0 0 0 % L
% Met: 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 34 0 0 0 0 0 0 0 0 0 0 0 7 0 % N
% Pro: 0 7 7 0 14 0 14 20 7 7 0 34 27 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 7 0 0 0 7 7 0 0 0 0 % R
% Ser: 60 0 20 7 14 47 7 40 0 7 40 20 47 7 7 % S
% Thr: 0 0 7 0 7 7 14 0 0 0 0 0 0 0 7 % T
% Val: 0 7 0 0 0 0 0 0 60 0 0 0 14 14 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _