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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2B All Species: 41.21
Human Site: S431 Identified Species: 64.76
UniProt: Q02880 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q02880 NP_001059.2 1626 183267 S431 S N C G I V E S I L N W V K F
Chimpanzee Pan troglodytes XP_516332 1634 184611 S439 S N C G I V E S I L N W V K F
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 S425 S N C G I V E S I L N W V K F
Dog Lupus familis XP_534241 2041 231068 S849 S N C G I V E S I L N W V K F
Cat Felis silvestris
Mouse Mus musculus Q64511 1612 181890 S419 S N C G I V E S I L N W V K F
Rat Rattus norvegicus P41516 1526 173202 S408 I G C G I V E S I L N W V K F
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001507216 1923 216719 S734 S N C G I V E S I L N W V K F
Chicken Gallus gallus O42131 1627 183228 S436 S N C G I I E S I L N W V K F
Frog Xenopus laevis NP_001082502 1579 178601 S408 T S C G I V E S I L N W V K F
Zebra Danio Brachydanio rerio NP_001038656 1618 182448 S430 T N C G I V E S I L N W V K F
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 L348 L W V F V N C L I E N P T F D
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 K421 E T M T L Q Q K Q F G S T C V
Sea Urchin Strong. purpuratus XP_783546 1448 163750 R349 I A T T K G G R H V D Y V S D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 A374 N A L I D N P A F D S Q T K E
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 S329 D Q I V K K I S E I L K K K K
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.3 98.5 78.1 N.A. 95.6 66.2 N.A. 79.3 89.2 66.6 73.6 N.A. 50.3 N.A. 49.5 57.3
Protein Similarity: 100 98.5 99.1 78.8 N.A. 97.2 78.1 N.A. 81.8 93.8 78.7 83.8 N.A. 64.3 N.A. 65.8 69.9
P-Site Identity: 100 100 100 100 N.A. 100 86.6 N.A. 100 93.3 86.6 93.3 N.A. 13.3 N.A. 0 6.6
P-Site Similarity: 100 100 100 100 N.A. 100 86.6 N.A. 100 100 100 100 N.A. 20 N.A. 13.3 26.6
Percent
Protein Identity: N.A. N.A. N.A. 43.3 40.7 N.A.
Protein Similarity: N.A. N.A. N.A. 59 56.8 N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 26.6 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 14 0 0 0 0 0 7 0 0 0 0 0 0 0 % A
% Cys: 0 0 67 0 0 0 7 0 0 0 0 0 0 7 0 % C
% Asp: 7 0 0 0 7 0 0 0 0 7 7 0 0 0 14 % D
% Glu: 7 0 0 0 0 0 67 0 7 7 0 0 0 0 7 % E
% Phe: 0 0 0 7 0 0 0 0 7 7 0 0 0 7 67 % F
% Gly: 0 7 0 67 0 7 7 0 0 0 7 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 % H
% Ile: 14 0 7 7 67 7 7 0 74 7 0 0 0 0 0 % I
% Lys: 0 0 0 0 14 7 0 7 0 0 0 7 7 80 7 % K
% Leu: 7 0 7 0 7 0 0 7 0 67 7 0 0 0 0 % L
% Met: 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 7 54 0 0 0 14 0 0 0 0 74 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 7 0 0 0 0 7 0 0 0 % P
% Gln: 0 7 0 0 0 7 7 0 7 0 0 7 0 0 0 % Q
% Arg: 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 % R
% Ser: 47 7 0 0 0 0 0 74 0 0 7 7 0 7 0 % S
% Thr: 14 7 7 14 0 0 0 0 0 0 0 0 20 0 0 % T
% Val: 0 0 7 7 7 60 0 0 0 7 0 0 74 0 7 % V
% Trp: 0 7 0 0 0 0 0 0 0 0 0 67 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _