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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2B All Species: 25.15
Human Site: S74 Identified Species: 39.52
UniProt: Q02880 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q02880 NP_001059.2 1626 183267 S74 R P D T Y I G S V E P L T Q F
Chimpanzee Pan troglodytes XP_516332 1634 184611 S82 R P D T Y I G S V E P L T Q F
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 T74 I G S V E P L T Q F M W V Y D
Dog Lupus familis XP_534241 2041 231068 S492 R P D T Y I G S V E P L T Q L
Cat Felis silvestris
Mouse Mus musculus Q64511 1612 181890 V72 P L T Q L M W V Y D E D V G M
Rat Rattus norvegicus P41516 1526 173202 R72 E D V G I N Y R E V T F V P G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001507216 1923 216719 S377 R P D T Y I G S V E P L T Q L
Chicken Gallus gallus O42131 1627 183228 S79 R P D T Y I G S V E P L T Q L
Frog Xenopus laevis NP_001082502 1579 178601 D72 D E G L N C R D I T F V P G L
Zebra Danio Brachydanio rerio NP_001038656 1618 182448 S73 R P D T Y I G S V E P V T Q Q
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 R26 Q L E H I L L R P D S Y I G S
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 S86 R P D T Y I G S V E H T E K T
Sea Urchin Strong. purpuratus XP_783546 1448 163750 K27 A A K K E A A K K R P L N D D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 I52 L L R P D T Y I G S I E K H T
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.3 98.5 78.1 N.A. 95.6 66.2 N.A. 79.3 89.2 66.6 73.6 N.A. 50.3 N.A. 49.5 57.3
Protein Similarity: 100 98.5 99.1 78.8 N.A. 97.2 78.1 N.A. 81.8 93.8 78.7 83.8 N.A. 64.3 N.A. 65.8 69.9
P-Site Identity: 100 100 0 93.3 N.A. 0 0 N.A. 93.3 93.3 0 86.6 N.A. 0 N.A. 66.6 13.3
P-Site Similarity: 100 100 6.6 93.3 N.A. 13.3 0 N.A. 93.3 93.3 13.3 93.3 N.A. 26.6 N.A. 73.3 13.3
Percent
Protein Identity: N.A. N.A. N.A. 43.3 40.7 N.A.
Protein Similarity: N.A. N.A. N.A. 59 56.8 N.A.
P-Site Identity: N.A. N.A. N.A. 0 0 N.A.
P-Site Similarity: N.A. N.A. N.A. 0 0 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 7 0 0 0 7 7 0 0 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 % C
% Asp: 7 7 47 0 7 0 0 7 0 14 0 7 0 7 14 % D
% Glu: 7 7 7 0 14 0 0 0 7 47 7 7 7 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 7 7 7 0 0 14 % F
% Gly: 0 7 7 7 0 0 47 0 7 0 0 0 0 20 7 % G
% His: 0 0 0 7 0 0 0 0 0 0 7 0 0 7 0 % H
% Ile: 7 0 0 0 14 47 0 7 7 0 7 0 7 0 0 % I
% Lys: 0 0 7 7 0 0 0 7 7 0 0 0 7 7 0 % K
% Leu: 7 20 0 7 7 7 14 0 0 0 0 40 0 0 27 % L
% Met: 0 0 0 0 0 7 0 0 0 0 7 0 0 0 7 % M
% Asn: 0 0 0 0 7 7 0 0 0 0 0 0 7 0 0 % N
% Pro: 7 47 0 7 0 7 0 0 7 0 47 0 7 7 0 % P
% Gln: 7 0 0 7 0 0 0 0 7 0 0 0 0 40 7 % Q
% Arg: 47 0 7 0 0 0 7 14 0 7 0 0 0 0 0 % R
% Ser: 0 0 7 0 0 0 0 47 0 7 7 0 0 0 7 % S
% Thr: 0 0 7 47 0 7 0 7 0 7 7 7 40 0 14 % T
% Val: 0 0 7 7 0 0 0 7 47 7 0 14 20 0 0 % V
% Trp: 0 0 0 0 0 0 7 0 0 0 0 7 0 0 0 % W
% Tyr: 0 0 0 0 47 0 14 0 7 0 0 7 0 7 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _