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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PRPF4B All Species: 25.76
Human Site: S427 Identified Species: 47.22
UniProt: Q13523 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.25
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q13523 NP_003904.3 1007 116973 S427 I L S R R E R S K D A S P I N
Chimpanzee Pan troglodytes XP_518214 1087 126250 S507 I L S R R E R S K D A S P I N
Rhesus Macaque Macaca mulatta XP_001094163 1007 116833 S427 I L S R R E R S K D A S P I N
Dog Lupus familis XP_848551 1042 120804 S461 L L S R R E R S K D A S P L N
Cat Felis silvestris
Mouse Mus musculus Q61136 1007 116929 S427 I L G R C E R S K D A S P I N
Rat Rattus norvegicus Q5RKH1 1007 116988 S427 I L G R C E R S K D A S P I N
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001508424 1011 117270 S430 I L S R R E R S K D V S P I S
Chicken Gallus gallus XP_001232309 1005 116507 K426 L S R R E R S K D I S P P S R
Frog Xenopus laevis NP_001088314 991 115012 A412 T A A R H D R A K E A S P S R
Zebra Danio Brachydanio rerio NP_998614 1010 116034 V443 T T P V R R R V S R S P L R R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_612010 907 104024 R410 T D N G D R D R Y K G S L S E
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans NP_492008 775 90931 I320 G A S T P V S I Q S D N D T P
Sea Urchin Strong. purpuratus XP_791787 924 105587 R397 R G R S P L E R R I R S S R S
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 92.6 99 95.3 N.A. 95.1 95.4 N.A. 93 89.7 80.6 68.8 N.A. 41.4 N.A. 36.8 48.3
Protein Similarity: 100 92.6 99.3 96.1 N.A. 97.5 97.8 N.A. 96.5 94 89.4 78.4 N.A. 57.4 N.A. 51.9 59.9
P-Site Identity: 100 100 100 86.6 N.A. 86.6 86.6 N.A. 86.6 13.3 40 13.3 N.A. 6.6 N.A. 6.6 6.6
P-Site Similarity: 100 100 100 100 N.A. 86.6 86.6 N.A. 93.3 26.6 66.6 20 N.A. 13.3 N.A. 20 20
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 16 8 0 0 0 0 8 0 0 54 0 0 0 0 % A
% Cys: 0 0 0 0 16 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 8 0 0 8 8 8 0 8 54 8 0 8 0 0 % D
% Glu: 0 0 0 0 8 54 8 0 0 8 0 0 0 0 8 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 8 8 16 8 0 0 0 0 0 0 8 0 0 0 0 % G
% His: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 47 0 0 0 0 0 0 8 0 16 0 0 0 47 0 % I
% Lys: 0 0 0 0 0 0 0 8 62 8 0 0 0 0 0 % K
% Leu: 16 54 0 0 0 8 0 0 0 0 0 0 16 8 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 8 0 0 0 0 0 0 0 0 8 0 0 47 % N
% Pro: 0 0 8 0 16 0 0 0 0 0 0 16 70 0 8 % P
% Gln: 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 % Q
% Arg: 8 0 16 70 47 24 70 16 8 8 8 0 0 16 24 % R
% Ser: 0 8 47 8 0 0 16 54 8 8 16 77 8 24 16 % S
% Thr: 24 8 0 8 0 0 0 0 0 0 0 0 0 8 0 % T
% Val: 0 0 0 8 0 8 0 8 0 0 8 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _