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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: EIF2B1 All Species: 43.94
Human Site: Y227 Identified Species: 80.56
UniProt: Q14232 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q14232 NP_001405.1 305 33712 Y227 K A Q N K P F Y V V A E S F K
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta XP_001101480 305 33696 Y227 K A Q N K P F Y A V A E S F K
Dog Lupus familis XP_534645 305 33854 Y227 K A Q N K P F Y V V A E S F K
Cat Felis silvestris
Mouse Mus musculus Q99LC8 305 33798 Y227 K A Q N K P F Y V V A E S F K
Rat Rattus norvegicus Q64270 305 33660 Y227 K A Q N K P F Y V V A E S F K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001507558 316 34903 Y238 K A Q N K L F Y V V A E S F K
Chicken Gallus gallus
Frog Xenopus laevis NP_001084600 302 33557 Y227 K A Q N K P F Y V V A E S F K
Zebra Danio Brachydanio rerio XP_002662251 302 33553 Y227 K A H N K P F Y V V A E S F K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_651752 306 34376 R227 Y T M G L C A R E M K K P F Y
Honey Bee Apis mellifera XP_392643 301 33602 Y228 K E V K K P F Y V L T E S F K
Nematode Worm Caenorhab. elegans P34604 305 34099 Y231 K S R H V P V Y V C A E T I K
Sea Urchin Strong. purpuratus XP_783380 304 33798 Y228 K V V N K P V Y V V A E S F K
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P14741 305 34007 Y228 H N A R K P F Y V V T E S H K
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. 99.3 96.3 N.A. 94 92.7 N.A. 88.2 N.A. 78 76.3 N.A. 49.6 53.4 43.2 59.3
Protein Similarity: 100 N.A. 99.3 97.3 N.A. 96.7 95 N.A. 92.7 N.A. 88.5 87.2 N.A. 67.6 75.7 63.9 75.7
P-Site Identity: 100 N.A. 93.3 100 N.A. 100 100 N.A. 93.3 N.A. 100 93.3 N.A. 6.6 66.6 46.6 80
P-Site Similarity: 100 N.A. 93.3 100 N.A. 100 100 N.A. 93.3 N.A. 100 93.3 N.A. 20 73.3 73.3 80
Percent
Protein Identity: N.A. N.A. N.A. N.A. 42.6 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 59.6 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 60 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 60 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 62 8 0 0 0 8 0 8 0 77 0 0 0 0 % A
% Cys: 0 0 0 0 0 8 0 0 0 8 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 0 8 0 0 0 0 0 0 8 0 0 93 0 0 0 % E
% Phe: 0 0 0 0 0 0 77 0 0 0 0 0 0 85 0 % F
% Gly: 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 8 0 8 8 0 0 0 0 0 0 0 0 0 8 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % I
% Lys: 85 0 0 8 85 0 0 0 0 0 8 8 0 0 93 % K
% Leu: 0 0 0 0 8 8 0 0 0 8 0 0 0 0 0 % L
% Met: 0 0 8 0 0 0 0 0 0 8 0 0 0 0 0 % M
% Asn: 0 8 0 70 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 85 0 0 0 0 0 0 8 0 0 % P
% Gln: 0 0 54 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 8 8 0 0 0 8 0 0 0 0 0 0 0 % R
% Ser: 0 8 0 0 0 0 0 0 0 0 0 0 85 0 0 % S
% Thr: 0 8 0 0 0 0 0 0 0 0 16 0 8 0 0 % T
% Val: 0 8 16 0 8 0 16 0 85 77 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 8 0 0 0 0 0 0 93 0 0 0 0 0 0 8 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _