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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: EIF4A2 All Species: 53.94
Human Site: S323 Identified Species: 79.11
UniProt: Q14240 Number Species: 15
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q14240 NP_001958.2 407 46402 S323 M R E F R S G S S R V L I T T
Chimpanzee Pan troglodytes A5A6N4 406 46165 S322 M R E F R S G S S R V L I T T
Rhesus Macaque Macaca mulatta XP_001102692 380 43227 M307 V S A L H G D M D Q K E R D V
Dog Lupus familis XP_860391 360 41029 D287 L N T R R K V D W L T E K M H
Cat Felis silvestris
Mouse Mus musculus P10630 407 46384 S323 M R E F R S G S S R V L I T T
Rat Rattus norvegicus NP_001008336 407 46384 S323 M R E F R S G S S R V L I T T
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus Q8JFP1 407 46353 S323 M R E F R S G S S R V L I T T
Frog Xenopus laevis NP_001085314 406 46000 S322 M R E F R S G S S R V L I T T
Zebra Danio Brachydanio rerio Q7ZVA6 406 46449 A322 M K E F R S G A S R V L I S T
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q02748 403 45860 S319 M K Q F R S G S S R V L I T T
Honey Bee Apis mellifera XP_623285 423 48137 S339 M R Q F R T G S S R V L I T T
Nematode Worm Caenorhab. elegans P27639 402 45389 S318 M R E F R S G S S R V L I T T
Sea Urchin Strong. purpuratus NP_001091916 426 48260 S342 M K E F R T G S S R V L I C T
Poplar Tree Populus trichocarpa
Maize Zea mays Q41741 410 46518 S326 M R E F R S G S S R V L I T T
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P41376 412 46686 S328 M R E F R S G S S R V L I T T
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7RV88 397 44826 S313 M K E F R S G S S R V L I A T
Conservation
Percent
Protein Identity: 100 89.4 93.1 88.4 N.A. 100 100 N.A. N.A. 97.7 90.4 67 N.A. 71.7 75.1 69.2 75.1
Protein Similarity: 100 96 93.3 88.4 N.A. 100 100 N.A. N.A. 99.5 95.8 80.3 N.A. 86.9 87.2 83 84
P-Site Identity: 100 100 0 6.6 N.A. 100 100 N.A. N.A. 100 100 80 N.A. 86.6 86.6 100 80
P-Site Similarity: 100 100 13.3 13.3 N.A. 100 100 N.A. N.A. 100 100 100 N.A. 100 100 100 93.3
Percent
Protein Identity: N.A. 68.7 N.A. 68.6 N.A. 74.9
Protein Similarity: N.A. 83.1 N.A. 82 N.A. 86.9
P-Site Identity: N.A. 100 N.A. 100 N.A. 86.6
P-Site Similarity: N.A. 100 N.A. 100 N.A. 93.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 0 0 0 7 0 0 0 0 0 7 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 % C
% Asp: 0 0 0 0 0 0 7 7 7 0 0 0 0 7 0 % D
% Glu: 0 0 75 0 0 0 0 0 0 0 0 13 0 0 0 % E
% Phe: 0 0 0 88 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 7 88 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 7 0 0 0 0 0 0 0 0 0 7 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 88 0 0 % I
% Lys: 0 25 0 0 0 7 0 0 0 0 7 0 7 0 0 % K
% Leu: 7 0 0 7 0 0 0 0 0 7 0 88 0 0 0 % L
% Met: 88 0 0 0 0 0 0 7 0 0 0 0 0 7 0 % M
% Asn: 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 13 0 0 0 0 0 0 7 0 0 0 0 0 % Q
% Arg: 0 63 0 7 94 0 0 0 0 88 0 0 7 0 0 % R
% Ser: 0 7 0 0 0 75 0 82 88 0 0 0 0 7 0 % S
% Thr: 0 0 7 0 0 13 0 0 0 0 7 0 0 69 88 % T
% Val: 7 0 0 0 0 0 7 0 0 0 88 0 0 0 7 % V
% Trp: 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _