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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MELK All Species: 31.52
Human Site: S253 Identified Species: 49.52
UniProt: Q14680 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q14680 NP_055606.1 651 74642 S253 V D P K K R I S M K N L L N H
Chimpanzee Pan troglodytes XP_001168921 651 74605 S253 V D P K K R I S M K N L L N H
Rhesus Macaque Macaca mulatta XP_001115076 651 74642 S253 V D P K K R I S M K N L L N H
Dog Lupus familis XP_538730 651 74149 S253 V D P K K R I S M K N L L S H
Cat Felis silvestris
Mouse Mus musculus Q61846 643 72695 P248 Q Q M L Q V D P K K R I S M R
Rat Rattus norvegicus P54645 559 63955 F180 N M M S D G E F L R T S C G S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509217 655 73776 S255 V D P K K R I S I K H L L N H
Chicken Gallus gallus NP_001026680 657 74208 T255 V D P K K R I T V K H L L S H
Frog Xenopus laevis NP_001081569 651 74289 T255 V D P K K R I T V K H L L N H
Zebra Danio Brachydanio rerio Q6NSM8 1187 130949 W309 E Q I C K N K W M R Q G D P D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_395000 606 68302 D227 K I L S G K Y D E P S W L S S
Nematode Worm Caenorhab. elegans NP_001023420 703 79966 S255 V V P E R R I S V K K L L E H
Sea Urchin Strong. purpuratus XP_781767 971 109658 K529 V N P T K R I K M K E L I I H
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q2V452 441 50581 R61 K M A E Q I R R E I A T M K L
Baker's Yeast Sacchar. cerevisiae P06782 633 72027 Y243 W S C G V I L Y V M L C R R L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 96.4 91.7 N.A. 83.4 29.6 N.A. 70.8 61.7 66.2 20.2 N.A. N.A. 39.9 37.9 32.7
Protein Similarity: 100 99.5 97.5 95.8 N.A. 90.3 47.4 N.A. 80.1 75.9 80.3 34.1 N.A. N.A. 55.7 56 45.5
P-Site Identity: 100 100 100 93.3 N.A. 6.6 0 N.A. 86.6 73.3 80 13.3 N.A. N.A. 6.6 60 60
P-Site Similarity: 100 100 100 100 N.A. 20 13.3 N.A. 100 100 100 20 N.A. N.A. 26.6 80 73.3
Percent
Protein Identity: N.A. N.A. N.A. 28.8 27 N.A.
Protein Similarity: N.A. N.A. N.A. 44.5 47.1 N.A.
P-Site Identity: N.A. N.A. N.A. 0 0 N.A.
P-Site Similarity: N.A. N.A. N.A. 20 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 0 0 0 0 0 0 7 0 0 0 0 % A
% Cys: 0 0 7 7 0 0 0 0 0 0 0 7 7 0 0 % C
% Asp: 0 47 0 0 7 0 7 7 0 0 0 0 7 0 7 % D
% Glu: 7 0 0 14 0 0 7 0 14 0 7 0 0 7 0 % E
% Phe: 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 7 7 7 0 0 0 0 0 7 0 7 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 20 0 0 0 60 % H
% Ile: 0 7 7 0 0 14 60 0 7 7 0 7 7 7 0 % I
% Lys: 14 0 0 47 60 7 7 7 7 67 7 0 0 7 0 % K
% Leu: 0 0 7 7 0 0 7 0 7 0 7 60 60 0 14 % L
% Met: 0 14 14 0 0 0 0 0 40 7 0 0 7 7 0 % M
% Asn: 7 7 0 0 0 7 0 0 0 0 27 0 0 34 0 % N
% Pro: 0 0 60 0 0 0 0 7 0 7 0 0 0 7 0 % P
% Gln: 7 14 0 0 14 0 0 0 0 0 7 0 0 0 0 % Q
% Arg: 0 0 0 0 7 60 7 7 0 14 7 0 7 7 7 % R
% Ser: 0 7 0 14 0 0 0 40 0 0 7 7 7 20 14 % S
% Thr: 0 0 0 7 0 0 0 14 0 0 7 7 0 0 0 % T
% Val: 60 7 0 0 7 7 0 0 27 0 0 0 0 0 0 % V
% Trp: 7 0 0 0 0 0 0 7 0 0 0 7 0 0 0 % W
% Tyr: 0 0 0 0 0 0 7 7 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _