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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MELK All Species: 11.82
Human Site: S335 Identified Species: 18.57
UniProt: Q14680 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q14680 NP_055606.1 651 74642 S335 K P V R L R L S S F S C G Q A
Chimpanzee Pan troglodytes XP_001168921 651 74605 S335 K P V H L R L S S F S C G Q A
Rhesus Macaque Macaca mulatta XP_001115076 651 74642 S335 K P V R L R L S S F S C G Q A
Dog Lupus familis XP_538730 651 74149 P335 K P V R L R L P S F S C G L A
Cat Felis silvestris
Mouse Mus musculus Q61846 643 72695 G327 L L A K K A R G K P A R L Q L
Rat Rattus norvegicus P54645 559 63955 M259 V I S L L K H M L Q V D P M K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509217 655 73776 T339 I R L R L L S T F H G Q T G F
Chicken Gallus gallus NP_001026680 657 74208 G341 L R I P S Q T G H A S T T Q S
Frog Xenopus laevis NP_001081569 651 74289 K335 H G K A V R L K H P L A V G D
Zebra Danio Brachydanio rerio Q6NSM8 1187 130949 C592 Q R S S Y K D C N T L H L P M
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_395000 606 68302 K306 N I W K K L V K S D R T D Y K
Nematode Worm Caenorhab. elegans NP_001023420 703 79966 S388 I S S S S D I S A R L K K N C
Sea Urchin Strong. purpuratus XP_781767 971 109658 S655 T S S P Y M F S S M E D G L E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q2V452 441 50581 P140 V Y H R D L K P E N L L L D S
Baker's Yeast Sacchar. cerevisiae P06782 633 72027 N327 K P H P E E E N E N N D S K K
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 96.4 91.7 N.A. 83.4 29.6 N.A. 70.8 61.7 66.2 20.2 N.A. N.A. 39.9 37.9 32.7
Protein Similarity: 100 99.5 97.5 95.8 N.A. 90.3 47.4 N.A. 80.1 75.9 80.3 34.1 N.A. N.A. 55.7 56 45.5
P-Site Identity: 100 93.3 100 86.6 N.A. 6.6 6.6 N.A. 13.3 13.3 13.3 0 N.A. N.A. 6.6 6.6 20
P-Site Similarity: 100 93.3 100 86.6 N.A. 20 13.3 N.A. 26.6 33.3 20 20 N.A. N.A. 20 20 20
Percent
Protein Identity: N.A. N.A. N.A. 28.8 27 N.A.
Protein Similarity: N.A. N.A. N.A. 44.5 47.1 N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 13.3 33.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 7 0 7 0 0 7 7 7 7 0 0 27 % A
% Cys: 0 0 0 0 0 0 0 7 0 0 0 27 0 0 7 % C
% Asp: 0 0 0 0 7 7 7 0 0 7 0 20 7 7 7 % D
% Glu: 0 0 0 0 7 7 7 0 14 0 7 0 0 0 7 % E
% Phe: 0 0 0 0 0 0 7 0 7 27 0 0 0 0 7 % F
% Gly: 0 7 0 0 0 0 0 14 0 0 7 0 34 14 0 % G
% His: 7 0 14 7 0 0 7 0 14 7 0 7 0 0 0 % H
% Ile: 14 14 7 0 0 0 7 0 0 0 0 0 0 0 0 % I
% Lys: 34 0 7 14 14 14 7 14 7 0 0 7 7 7 20 % K
% Leu: 14 7 7 7 40 20 34 0 7 0 27 7 20 14 7 % L
% Met: 0 0 0 0 0 7 0 7 0 7 0 0 0 7 7 % M
% Asn: 7 0 0 0 0 0 0 7 7 14 7 0 0 7 0 % N
% Pro: 0 34 0 20 0 0 0 14 0 14 0 0 7 7 0 % P
% Gln: 7 0 0 0 0 7 0 0 0 7 0 7 0 34 0 % Q
% Arg: 0 20 0 34 0 34 7 0 0 7 7 7 0 0 0 % R
% Ser: 0 14 27 14 14 0 7 34 40 0 34 0 7 0 14 % S
% Thr: 7 0 0 0 0 0 7 7 0 7 0 14 14 0 0 % T
% Val: 14 0 27 0 7 0 7 0 0 0 7 0 7 0 0 % V
% Trp: 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 7 0 0 14 0 0 0 0 0 0 0 0 7 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _