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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PPP2R4 All Species: 5.45
Human Site: T65 Identified Species: 9.23
UniProt: Q15257 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.31
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q15257 NP_066954.2 358 40668 T65 G V K G K K L T F E Y R V S E
Chimpanzee Pan troglodytes XP_001163459 373 41748 S63 R Q P P P D S S E E A P P A T
Rhesus Macaque Macaca mulatta XP_001108890 359 40767 T65 G V K G K K L T F E Y K V S E
Dog Lupus familis XP_548424 349 39477 M62 E I H T V P D M G K W K R S Q
Cat Felis silvestris
Mouse Mus musculus P58389 323 36692 K62 L N E G V K G K K L T F D Y K
Rat Rattus norvegicus NP_001102047 323 36599 K62 L N E G V K G K K L T F D Y K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001508723 358 40562 N67 K I P K K E I N M V P D M G K
Chicken Gallus gallus NP_001026542 321 36721 R61 L N E G V K G R K L T C E Y N
Frog Xenopus laevis NP_001089500 323 36857 K61 L A L N E A V K G K K L T D D
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_523466 398 44238 L67 S E M L K K L L R L F D A L E
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_784258 466 51558 L124 Y S R A T V A L D Y D R L T D
Poplar Tree Populus trichocarpa XP_002307365 396 45132 L63 I R S P A I N L P P N T N S Q
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P40454 393 45064 I64 P H A T A S S I A M V N G L M
Red Bread Mold Neurospora crassa Q7SEF9 449 50080 V74 R P D P S T L V F Q T P V R R
Conservation
Percent
Protein Identity: 100 72.1 93.3 73.4 N.A. 86.8 85.7 N.A. 68.7 81.2 76.2 N.A. N.A. 41.2 N.A. N.A. 39
Protein Similarity: 100 76.9 96 77.3 N.A. 89.6 89.1 N.A. 75.9 85.1 82.6 N.A. N.A. 56 N.A. N.A. 50.4
P-Site Identity: 100 6.6 93.3 6.6 N.A. 13.3 13.3 N.A. 6.6 13.3 0 N.A. N.A. 26.6 N.A. N.A. 6.6
P-Site Similarity: 100 20 100 40 N.A. 26.6 26.6 N.A. 40 20 26.6 N.A. N.A. 33.3 N.A. N.A. 33.3
Percent
Protein Identity: 38.3 N.A. N.A. N.A. 36.1 30.2
Protein Similarity: 56.8 N.A. N.A. N.A. 50.3 43.8
P-Site Identity: 6.6 N.A. N.A. N.A. 0 20
P-Site Similarity: 13.3 N.A. N.A. N.A. 0 26.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 8 8 15 8 8 0 8 0 8 0 8 8 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 % C
% Asp: 0 0 8 0 0 8 8 0 8 0 8 15 15 8 15 % D
% Glu: 8 8 22 0 8 8 0 0 8 22 0 0 8 0 22 % E
% Phe: 0 0 0 0 0 0 0 0 22 0 8 15 0 0 0 % F
% Gly: 15 0 0 36 0 0 22 0 15 0 0 0 8 8 0 % G
% His: 0 8 8 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 8 15 0 0 0 8 8 8 0 0 0 0 0 0 0 % I
% Lys: 8 0 15 8 29 43 0 22 22 15 8 15 0 0 22 % K
% Leu: 29 0 8 8 0 0 29 22 0 29 0 8 8 15 0 % L
% Met: 0 0 8 0 0 0 0 8 8 8 0 0 8 0 8 % M
% Asn: 0 22 0 8 0 0 8 8 0 0 8 8 8 0 8 % N
% Pro: 8 8 15 22 8 8 0 0 8 8 8 15 8 0 0 % P
% Gln: 0 8 0 0 0 0 0 0 0 8 0 0 0 0 15 % Q
% Arg: 15 8 8 0 0 0 0 8 8 0 0 15 8 8 8 % R
% Ser: 8 8 8 0 8 8 15 8 0 0 0 0 0 29 0 % S
% Thr: 0 0 0 15 8 8 0 15 0 0 29 8 8 8 8 % T
% Val: 0 15 0 0 29 8 8 8 0 8 8 0 22 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0 % W
% Tyr: 8 0 0 0 0 0 0 0 0 8 15 0 0 22 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _