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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: E2F4 All Species: 11.52
Human Site: S285 Identified Species: 23.03
UniProt: Q16254 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q16254 NP_001941.2 413 43960 S285 S K D S G E L S S L P L G P T
Chimpanzee Pan troglodytes XP_511025 426 45548 S304 S K D S G E L S S L P L G P T
Rhesus Macaque Macaca mulatta XP_001088067 416 44276 S288 K D S G E L S S L P L G P T A
Dog Lupus familis XP_853975 409 43531 G286 S K D G G E L G S L P P G L A
Cat Felis silvestris
Mouse Mus musculus Q8R0K9 410 43815 S286 N K D S G E V S S L P L G L T
Rat Rattus norvegicus Q62814 300 33206 I179 L K S H S G P I H V L L I N K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001506812 384 40812 P259 L D S S S V L P H P S T S F E
Chicken Gallus gallus XP_001231948 414 44371 V286 S T E C S I S V V E S K S S S
Frog Xenopus laevis NP_001086706 375 40890 C254 S N A E S Q D C P T G Q T F S
Zebra Danio Brachydanio rerio NP_998597 393 42365 T265 T T T V P P N T N S G P P A V
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624285 300 34327 N178 E E D D K E I N Y E M F L K S
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_799123 356 39484 S235 E P I A Q A I S A P S V V V Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 94.3 97.5 93.4 N.A. 92.4 52.2 N.A. 37 71.2 67.8 63.4 N.A. N.A. 38 N.A. 50.6
Protein Similarity: 100 94.5 98.5 94.4 N.A. 95.1 62.4 N.A. 47.7 78.7 77.9 74.8 N.A. N.A. 53.7 N.A. 63.4
P-Site Identity: 100 100 6.6 66.6 N.A. 80 13.3 N.A. 13.3 6.6 6.6 0 N.A. N.A. 13.3 N.A. 6.6
P-Site Similarity: 100 100 6.6 66.6 N.A. 93.3 20 N.A. 13.3 20 20 20 N.A. N.A. 40 N.A. 33.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 9 9 0 9 0 0 9 0 0 0 0 9 17 % A
% Cys: 0 0 0 9 0 0 0 9 0 0 0 0 0 0 0 % C
% Asp: 0 17 42 9 0 0 9 0 0 0 0 0 0 0 0 % D
% Glu: 17 9 9 9 9 42 0 0 0 17 0 0 0 0 9 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 9 0 17 0 % F
% Gly: 0 0 0 17 34 9 0 9 0 0 17 9 34 0 0 % G
% His: 0 0 0 9 0 0 0 0 17 0 0 0 0 0 0 % H
% Ile: 0 0 9 0 0 9 17 9 0 0 0 0 9 0 0 % I
% Lys: 9 42 0 0 9 0 0 0 0 0 0 9 0 9 9 % K
% Leu: 17 0 0 0 0 9 34 0 9 34 17 34 9 17 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 % M
% Asn: 9 9 0 0 0 0 9 9 9 0 0 0 0 9 0 % N
% Pro: 0 9 0 0 9 9 9 9 9 25 34 17 17 17 0 % P
% Gln: 0 0 0 0 9 9 0 0 0 0 0 9 0 0 9 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 42 0 25 34 34 0 17 42 34 9 25 0 17 9 25 % S
% Thr: 9 17 9 0 0 0 0 9 0 9 0 9 9 9 25 % T
% Val: 0 0 0 9 0 9 9 9 9 9 0 9 9 9 9 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _