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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: E2F4 All Species: 10.91
Human Site: T248 Identified Species: 21.82
UniProt: Q16254 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q16254 NP_001941.2 413 43960 T248 R P N S P Q L T P T A V P G S
Chimpanzee Pan troglodytes XP_511025 426 45548 T267 R P N S P Q L T P T A V P G S
Rhesus Macaque Macaca mulatta XP_001088067 416 44276 P251 P N S P Q L T P T P V P G S A
Dog Lupus familis XP_853975 409 43531 T249 P S S P Q L T T P T P V T G I
Cat Felis silvestris
Mouse Mus musculus Q8R0K9 410 43815 T249 P S S P Q L T T P T P V L G S
Rat Rattus norvegicus Q62814 300 33206 L142 S C F N G D T L L A I Q A P S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001506812 384 40812 A222 S T Q V H G A A G Q A T A G T
Chicken Gallus gallus XP_001231948 414 44371 Q249 I P P L A H F Q E A S V P S S
Frog Xenopus laevis NP_001086706 375 40890 A217 D L I Q A P P A V P S T P Q R
Zebra Danio Brachydanio rerio NP_998597 393 42365 P228 T T S A A A A P T K P T A N S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_624285 300 34327 T141 E D V K E N F T D D F V L G I
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_799123 356 39484 S198 N K D E T S N S P V V V P V P
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 94.3 97.5 93.4 N.A. 92.4 52.2 N.A. 37 71.2 67.8 63.4 N.A. N.A. 38 N.A. 50.6
Protein Similarity: 100 94.5 98.5 94.4 N.A. 95.1 62.4 N.A. 47.7 78.7 77.9 74.8 N.A. N.A. 53.7 N.A. 63.4
P-Site Identity: 100 100 0 33.3 N.A. 40 6.6 N.A. 13.3 26.6 6.6 6.6 N.A. N.A. 20 N.A. 20
P-Site Similarity: 100 100 13.3 40 N.A. 46.6 13.3 N.A. 20 33.3 13.3 20 N.A. N.A. 20 N.A. 33.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 9 25 9 17 17 0 17 25 0 25 0 9 % A
% Cys: 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 9 9 9 0 0 9 0 0 9 9 0 0 0 0 0 % D
% Glu: 9 0 0 9 9 0 0 0 9 0 0 0 0 0 0 % E
% Phe: 0 0 9 0 0 0 17 0 0 0 9 0 0 0 0 % F
% Gly: 0 0 0 0 9 9 0 0 9 0 0 0 9 50 0 % G
% His: 0 0 0 0 9 9 0 0 0 0 0 0 0 0 0 % H
% Ile: 9 0 9 0 0 0 0 0 0 0 9 0 0 0 17 % I
% Lys: 0 9 0 9 0 0 0 0 0 9 0 0 0 0 0 % K
% Leu: 0 9 0 9 0 25 17 9 9 0 0 0 17 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 9 9 17 9 0 9 9 0 0 0 0 0 0 9 0 % N
% Pro: 25 25 9 25 17 9 9 17 42 17 25 9 42 9 9 % P
% Gln: 0 0 9 9 25 17 0 9 0 9 0 9 0 9 0 % Q
% Arg: 17 0 0 0 0 0 0 0 0 0 0 0 0 0 9 % R
% Ser: 17 17 34 17 0 9 0 9 0 0 17 0 0 17 50 % S
% Thr: 9 17 0 0 9 0 34 42 17 34 0 25 9 0 9 % T
% Val: 0 0 9 9 0 0 0 0 9 9 17 59 0 9 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _