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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKFB3 All Species: 23.33
Human Site: S431 Identified Species: 46.67
UniProt: Q16875 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0.18
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q16875 NP_001138915.1 520 59609 S431 A Y G C R V E S I Y L N V E S
Chimpanzee Pan troglodytes XP_001147864 523 59913 S431 A Y G C R V E S I Y L N V E S
Rhesus Macaque Macaca mulatta XP_001107535 520 59589 S431 A Y G C R V E S I Y L N V E S
Dog Lupus familis XP_544267 559 63551 S444 A Y G C R V E S I Y L N V E S
Cat Felis silvestris
Mouse Mus musculus P70265 519 59907 A430 I F K L T P V A Y G C K V E T
Rat Rattus norvegicus O35552 555 63657 S431 A Y G C R V E S I Y L N V E S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516067 579 65588 A490 V L K L T P V A Y G C R V E S
Chicken Gallus gallus Q91348 470 54386 E382 E P V I M E L E R Q E N V L V
Frog Xenopus laevis NP_001088535 470 54750 E382 E P V I M E L E Q Q E N V L V
Zebra Danio Brachydanio rerio NP_998562 536 62010 S434 A Y G C K V E S I S L N V E A
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q21122 457 52072 S369 E S Y E D V V S R L E P V I M
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P32604 452 52576 I364 E R Q E N V L I I T H Q A V L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 96.7 99.6 84.6 N.A. 65.5 89.9 N.A. 76.6 61.1 65.5 72.3 N.A. N.A. N.A. 47.1 N.A.
Protein Similarity: 100 97.1 99.8 87.6 N.A. 77.8 91.7 N.A. 84.2 77.1 76.7 84.1 N.A. N.A. N.A. 62.8 N.A.
P-Site Identity: 100 100 100 100 N.A. 13.3 100 N.A. 20 13.3 13.3 80 N.A. N.A. N.A. 20 N.A.
P-Site Similarity: 100 100 100 100 N.A. 33.3 100 N.A. 26.6 13.3 13.3 93.3 N.A. N.A. N.A. 20 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 40.9 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 59.4 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 50 0 0 0 0 0 0 17 0 0 0 0 9 0 9 % A
% Cys: 0 0 0 50 0 0 0 0 0 0 17 0 0 0 0 % C
% Asp: 0 0 0 0 9 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 34 0 0 17 0 17 50 17 0 0 25 0 0 67 0 % E
% Phe: 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 50 0 0 0 0 0 0 17 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 % H
% Ile: 9 0 0 17 0 0 0 9 59 0 0 0 0 9 0 % I
% Lys: 0 0 17 0 9 0 0 0 0 0 0 9 0 0 0 % K
% Leu: 0 9 0 17 0 0 25 0 0 9 50 0 0 17 9 % L
% Met: 0 0 0 0 17 0 0 0 0 0 0 0 0 0 9 % M
% Asn: 0 0 0 0 9 0 0 0 0 0 0 67 0 0 0 % N
% Pro: 0 17 0 0 0 17 0 0 0 0 0 9 0 0 0 % P
% Gln: 0 0 9 0 0 0 0 0 9 17 0 9 0 0 0 % Q
% Arg: 0 9 0 0 42 0 0 0 17 0 0 9 0 0 0 % R
% Ser: 0 9 0 0 0 0 0 59 0 9 0 0 0 0 50 % S
% Thr: 0 0 0 0 17 0 0 0 0 9 0 0 0 0 9 % T
% Val: 9 0 17 0 0 67 25 0 0 0 0 0 92 9 17 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 50 9 0 0 0 0 0 17 42 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _