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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKFB3 All Species: 15.76
Human Site: S8 Identified Species: 31.52
UniProt: Q16875 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q16875 NP_001138915.1 520 59609 S8 M P L E L T Q S R V Q K I W V
Chimpanzee Pan troglodytes XP_001147864 523 59913 S8 M P L E L T Q S R V Q K I W V
Rhesus Macaque Macaca mulatta XP_001107535 520 59589 S8 M P L E L T Q S R V Q K I W V
Dog Lupus familis XP_544267 559 63551 G21 Q N P K G R G G R R H T N P K
Cat Felis silvestris
Mouse Mus musculus P70265 519 59907 N14 F S T E D S C N S S Y K P H A
Rat Rattus norvegicus O35552 555 63657 S8 M P L E L T Q S R V Q K I W V
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516067 579 65588 T74 K V P L A L R T V L C K I Y I
Chicken Gallus gallus Q91348 470 54386 P13 S G Q L T Q N P L Q K V W V P
Frog Xenopus laevis NP_001088535 470 54750 L13 R E L K Q T R L Q K I W I P H
Zebra Danio Brachydanio rerio NP_998562 536 62010 Q11 E L T Q N R I Q K I W I P S K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q21122 457 52072
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P32604 452 52576
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 96.7 99.6 84.6 N.A. 65.5 89.9 N.A. 76.6 61.1 65.5 72.3 N.A. N.A. N.A. 47.1 N.A.
Protein Similarity: 100 97.1 99.8 87.6 N.A. 77.8 91.7 N.A. 84.2 77.1 76.7 84.1 N.A. N.A. N.A. 62.8 N.A.
P-Site Identity: 100 100 100 6.6 N.A. 13.3 100 N.A. 13.3 0 20 0 N.A. N.A. N.A. 0 N.A.
P-Site Similarity: 100 100 100 13.3 N.A. 26.6 100 N.A. 46.6 6.6 40 20 N.A. N.A. N.A. 0 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 40.9 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 59.4 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 0 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 0 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 9 0 0 0 0 0 0 0 0 0 9 % A
% Cys: 0 0 0 0 0 0 9 0 0 0 9 0 0 0 0 % C
% Asp: 0 0 0 0 9 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 9 9 0 42 0 0 0 0 0 0 0 0 0 0 0 % E
% Phe: 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 9 0 0 9 0 9 9 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 9 0 0 9 9 % H
% Ile: 0 0 0 0 0 0 9 0 0 9 9 9 50 0 9 % I
% Lys: 9 0 0 17 0 0 0 0 9 9 9 50 0 0 17 % K
% Leu: 0 9 42 17 34 9 0 9 9 9 0 0 0 0 0 % L
% Met: 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 9 0 0 9 0 9 9 0 0 0 0 9 0 0 % N
% Pro: 0 34 17 0 0 0 0 9 0 0 0 0 17 17 9 % P
% Gln: 9 0 9 9 9 9 34 9 9 9 34 0 0 0 0 % Q
% Arg: 9 0 0 0 0 17 17 0 42 9 0 0 0 0 0 % R
% Ser: 9 9 0 0 0 9 0 34 9 9 0 0 0 9 0 % S
% Thr: 0 0 17 0 9 42 0 9 0 0 0 9 0 0 0 % T
% Val: 0 9 0 0 0 0 0 0 9 34 0 9 0 9 34 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 9 9 9 34 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 9 0 0 9 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _