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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKFB3 All Species: 9.39
Human Site: T471 Identified Species: 18.79
UniProt: Q16875 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q16875 NP_001138915.1 520 59609 T471 P L A S P E P T K K P R I N S
Chimpanzee Pan troglodytes XP_001147864 523 59913 T471 P L A S P E P T K K P R I N S
Rhesus Macaque Macaca mulatta XP_001107535 520 59589 T471 P L A S P E P T K K P R I N S
Dog Lupus familis XP_544267 559 63551 L484 V P S S D L D L V S C R H G T
Cat Felis silvestris
Mouse Mus musculus P70265 519 59907 F470 V R M R R N S F T P L S S S N
Rat Rattus norvegicus O35552 555 63657 V471 T E L D F L S V E S A K Q D A
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516067 579 65588 P530 M R R N S V T P L A S P E P T
Chicken Gallus gallus Q91348 470 54386 L422 R C P L H T V L K L T P V A Y
Frog Xenopus laevis NP_001088535 470 54750 L422 K C P L H T V L K L T P V A Y
Zebra Danio Brachydanio rerio NP_998562 536 62010 F474 P L T S H E P F K K P R I D G
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q21122 457 52072 P409 D R P L S E L P Y I D I P L H
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P32604 452 52576 Y404 I K L E P R A Y G T K V T K I
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 96.7 99.6 84.6 N.A. 65.5 89.9 N.A. 76.6 61.1 65.5 72.3 N.A. N.A. N.A. 47.1 N.A.
Protein Similarity: 100 97.1 99.8 87.6 N.A. 77.8 91.7 N.A. 84.2 77.1 76.7 84.1 N.A. N.A. N.A. 62.8 N.A.
P-Site Identity: 100 100 100 13.3 N.A. 0 0 N.A. 0 6.6 6.6 66.6 N.A. N.A. N.A. 6.6 N.A.
P-Site Similarity: 100 100 100 26.6 N.A. 13.3 26.6 N.A. 13.3 13.3 13.3 73.3 N.A. N.A. N.A. 6.6 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 40.9 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 59.4 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 6.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 25 0 0 0 9 0 0 9 9 0 0 17 9 % A
% Cys: 0 17 0 0 0 0 0 0 0 0 9 0 0 0 0 % C
% Asp: 9 0 0 9 9 0 9 0 0 0 9 0 0 17 0 % D
% Glu: 0 9 0 9 0 42 0 0 9 0 0 0 9 0 0 % E
% Phe: 0 0 0 0 9 0 0 17 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 9 0 0 0 0 9 9 % G
% His: 0 0 0 0 25 0 0 0 0 0 0 0 9 0 9 % H
% Ile: 9 0 0 0 0 0 0 0 0 9 0 9 34 0 9 % I
% Lys: 9 9 0 0 0 0 0 0 50 34 9 9 0 9 0 % K
% Leu: 0 34 17 25 0 17 9 25 9 17 9 0 0 9 0 % L
% Met: 9 0 9 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 9 0 9 0 0 0 0 0 0 0 25 9 % N
% Pro: 34 9 25 0 34 0 34 17 0 9 34 25 9 9 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 % Q
% Arg: 9 25 9 9 9 9 0 0 0 0 0 42 0 0 0 % R
% Ser: 0 0 9 42 17 0 17 0 0 17 9 9 9 9 25 % S
% Thr: 9 0 9 0 0 17 9 25 9 9 17 0 9 0 17 % T
% Val: 17 0 0 0 0 9 17 9 9 0 0 9 17 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 9 9 0 0 0 0 0 17 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _