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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: AGK All Species: 19.09
Human Site: S274 Identified Species: 32.31
UniProt: Q53H12 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0.15
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q53H12 NP_060708.1 422 47137 S274 E T P V Q R P S L Y R R I L R
Chimpanzee Pan troglodytes XP_001156048 422 47094 S274 E T P V Q R P S L Y R R V L R
Rhesus Macaque Macaca mulatta XP_001083626 422 47078 S274 E T P V Q R P S L Y R R I L R
Dog Lupus familis XP_539880 421 46699 S273 E E T P P R P S L Y R R I L R
Cat Felis silvestris
Mouse Mus musculus Q9ESW4 421 46957 S273 E E T P P R P S L Y R R I L R
Rat Rattus norvegicus NP_001120969 421 46750 S273 E D A A P R P S L Y R R I L R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001515850 753 82083 P338 E E R P P R P P L Y R R L Y R
Chicken Gallus gallus XP_416354 424 47762 P276 E E K P S R P P L Y V R L Y R
Frog Xenopus laevis Q7ZYJ3 428 47944 P276 E Q K P S R P P L Y V R I Y R
Zebra Danio Brachydanio rerio Q7ZW00 422 47526 L277 E I P Q R P N L L Y R I Y R R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_723549 408 45697 E274 A A T V Q R Q E T A Q P S G L
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q10123 439 49955 E282 C R P K P I I E A P Q W R W W
Sea Urchin Strong. purpuratus XP_784956 377 42102 E243 R L W P K Q V E P E P E V Q E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9LRB0 763 83573 K553 N W P R T R S K S R M D K G W
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.2 98.5 91.9 N.A. 91.7 90.7 N.A. 42.9 74.5 68.9 60.4 N.A. 25.1 N.A. 22.1 33.6
Protein Similarity: 100 99.7 99.2 96.2 N.A. 95.2 94 N.A. 49.5 85.8 82.4 75.3 N.A. 44.5 N.A. 39.8 54.5
P-Site Identity: 100 93.3 100 73.3 N.A. 73.3 73.3 N.A. 53.3 46.6 53.3 40 N.A. 20 N.A. 6.6 0
P-Site Similarity: 100 100 100 73.3 N.A. 73.3 73.3 N.A. 60 53.3 53.3 46.6 N.A. 26.6 N.A. 13.3 20
Percent
Protein Identity: N.A. N.A. N.A. 20.9 N.A. N.A.
Protein Similarity: N.A. N.A. N.A. 32.7 N.A. N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. 13.3 N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 8 8 8 0 0 0 0 8 8 0 0 0 0 0 % A
% Cys: 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 8 0 0 0 0 0 0 0 0 0 8 0 0 0 % D
% Glu: 72 29 0 0 0 0 0 22 0 8 0 8 0 0 8 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 15 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 8 0 0 0 8 8 0 0 0 0 8 43 0 0 % I
% Lys: 0 0 15 8 8 0 0 8 0 0 0 0 8 0 0 % K
% Leu: 0 8 0 0 0 0 0 8 72 0 0 0 15 43 8 % L
% Met: 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0 % M
% Asn: 8 0 0 0 0 0 8 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 43 43 36 8 65 22 8 8 8 8 0 0 0 % P
% Gln: 0 8 0 8 29 8 8 0 0 0 15 0 0 8 0 % Q
% Arg: 8 8 8 8 8 79 0 0 0 8 58 65 8 8 72 % R
% Ser: 0 0 0 0 15 0 8 43 8 0 0 0 8 0 0 % S
% Thr: 0 22 22 0 8 0 0 0 8 0 0 0 0 0 0 % T
% Val: 0 0 0 29 0 0 8 0 0 0 15 0 15 0 0 % V
% Trp: 0 8 8 0 0 0 0 0 0 0 0 8 0 8 15 % W
% Tyr: 0 0 0 0 0 0 0 0 0 72 0 0 8 22 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _