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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: DEF All Species: 9.7
Human Site: S164 Identified Species: 17.78
UniProt: Q68CQ4 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q68CQ4 NP_055203.4 756 87055 S164 F T D A K H E S L F S L E T N
Chimpanzee Pan troglodytes XP_525053 756 87125 S164 F T D A K H E S L F S L E T N
Rhesus Macaque Macaca mulatta XP_001109922 755 86704 S163 F T D A K H E S L F S L E T N
Dog Lupus familis XP_547394 639 73475 K100 P R T T H Q L K W P V L G Q L
Cat Felis silvestris
Mouse Mus musculus Q8BTT6 772 88799 G164 K D G E E P P G V S Q K S S E
Rat Rattus norvegicus Q5M9G7 763 87806 D164 K S S E E F T D V K H E S L F
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509371 772 89238 F166 Q S G A E E D F T D V K H E S
Chicken Gallus gallus Q5ZLG3 772 88932 E162 D P N R G A I E E F T D V K H
Frog Xenopus laevis NP_001091284 767 88492 E165 P A A E D A G E F T D A K H E
Zebra Danio Brachydanio rerio Q6PEH4 753 86784 T162 A E M E G E F T D K K N E A A
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_611851 700 81542 A161 T I K R N W S A L G N V R F D
Honey Bee Apis mellifera XP_395073 700 81460 V160 L Y E A V S N V P Q I M E T Q
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P40498 721 83973 K172 H F N Q V P E K F V D K L S N
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.3 97.7 78 N.A. 86.2 87.8 N.A. 74.3 68.9 68 54.5 N.A. 35.3 42.2 N.A. N.A.
Protein Similarity: 100 99.7 98.4 81.3 N.A. 91.1 93.3 N.A. 85.6 81.3 82 71 N.A. 56.3 62.8 N.A. N.A.
P-Site Identity: 100 100 100 6.6 N.A. 0 0 N.A. 6.6 6.6 0 6.6 N.A. 6.6 20 N.A. N.A.
P-Site Similarity: 100 100 100 6.6 N.A. 20 20 N.A. 33.3 26.6 6.6 13.3 N.A. 33.3 33.3 N.A. N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 31.2 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 50 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 8 8 39 0 16 0 8 0 0 0 8 0 8 8 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 8 8 24 0 8 0 8 8 8 8 16 8 0 0 8 % D
% Glu: 0 8 8 31 24 16 31 16 8 0 0 8 39 8 16 % E
% Phe: 24 8 0 0 0 8 8 8 16 31 0 0 0 8 8 % F
% Gly: 0 0 16 0 16 0 8 8 0 8 0 0 8 0 0 % G
% His: 8 0 0 0 8 24 0 0 0 0 8 0 8 8 8 % H
% Ile: 0 8 0 0 0 0 8 0 0 0 8 0 0 0 0 % I
% Lys: 16 0 8 0 24 0 0 16 0 16 8 24 8 8 0 % K
% Leu: 8 0 0 0 0 0 8 0 31 0 0 31 8 8 8 % L
% Met: 0 0 8 0 0 0 0 0 0 0 0 8 0 0 0 % M
% Asn: 0 0 16 0 8 0 8 0 0 0 8 8 0 0 31 % N
% Pro: 16 8 0 0 0 16 8 0 8 8 0 0 0 0 0 % P
% Gln: 8 0 0 8 0 8 0 0 0 8 8 0 0 8 8 % Q
% Arg: 0 8 0 16 0 0 0 0 0 0 0 0 8 0 0 % R
% Ser: 0 16 8 0 0 8 8 24 0 8 24 0 16 16 8 % S
% Thr: 8 24 8 8 0 0 8 8 8 8 8 0 0 31 0 % T
% Val: 0 0 0 0 16 0 0 8 16 8 16 8 8 0 0 % V
% Trp: 0 0 0 0 0 8 0 0 8 0 0 0 0 0 0 % W
% Tyr: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _