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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 4.55
Human Site: S182 Identified Species: 7.14
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 S182 G P P E L S G S A S P S R Y H
Chimpanzee Pan troglodytes XP_512373 411 46398 H149 E L E Q E R R H R Q I V S W F
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 S182 G P P E L S G S A S P S R Y H
Dog Lupus familis XP_542069 473 52729 L181 G P S E P A G L A S P N R Y R
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 P182 A S S E M P G P A S P S R C R
Rat Rattus norvegicus NP_001094483 473 52805 P181 A S S E M P G P A S P S R Y R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 P174 G E R D P R P P S A S R N A P
Chicken Gallus gallus Q6PZ02 393 44512 M130 S I H Q I A Q M G V G E G K S
Frog Xenopus laevis Q68FJ9 469 52793 I169 H F V E M E P I R S S S P P S
Zebra Danio Brachydanio rerio Q6DG88 394 44435 G132 I H Q I A Q M G V G E G K S I
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 K350 W F G D S S S K S S P F S I H
Honey Bee Apis mellifera XP_393739 477 54008 Q202 D Q P I K T E Q Q K L D E Y N
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 G433 S L I L H F L G R E W N I Y K
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 N167 T T Y T S D V N W G C M I R S
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 A238 G Q S L L A N A I L I A R L G
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 0 100 60 N.A. 46.6 53.3 N.A. 6.6 0 20 0 N.A. 26.6 13.3 N.A. 6.6
P-Site Similarity: 100 13.3 100 73.3 N.A. 53.3 60 N.A. 26.6 20 26.6 6.6 N.A. 40 26.6 N.A. 13.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 20
P-Site Similarity: N.A. N.A. N.A. 6.6 N.A. 40
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 14 0 0 0 7 20 0 7 34 7 0 7 0 7 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 7 0 0 7 0 % C
% Asp: 7 0 0 14 0 7 0 0 0 0 0 7 0 0 0 % D
% Glu: 7 7 7 40 7 7 7 0 0 7 7 7 7 0 0 % E
% Phe: 0 14 0 0 0 7 0 0 0 0 0 7 0 0 7 % F
% Gly: 34 0 7 0 0 0 34 14 7 14 7 7 7 0 7 % G
% His: 7 7 7 0 7 0 0 7 0 0 0 0 0 0 20 % H
% Ile: 7 7 7 14 7 0 0 7 7 0 14 0 14 7 7 % I
% Lys: 0 0 0 0 7 0 0 7 0 7 0 0 7 7 7 % K
% Leu: 0 14 0 14 20 0 7 7 0 7 7 0 0 7 0 % L
% Met: 0 0 0 0 20 0 7 7 0 0 0 7 0 0 0 % M
% Asn: 0 0 0 0 0 0 7 7 0 0 0 14 7 0 7 % N
% Pro: 0 20 20 0 14 14 14 20 0 0 40 0 7 7 7 % P
% Gln: 0 14 7 14 0 7 7 7 7 7 0 0 0 0 0 % Q
% Arg: 0 0 7 0 0 14 7 0 20 0 0 7 40 7 20 % R
% Ser: 14 14 27 0 14 20 7 14 14 47 14 34 14 7 20 % S
% Thr: 7 7 0 7 0 7 0 0 0 0 0 0 0 0 0 % T
% Val: 0 0 7 0 0 0 7 0 7 7 0 7 0 0 0 % V
% Trp: 7 0 0 0 0 0 0 0 7 0 7 0 0 7 0 % W
% Tyr: 0 0 7 0 0 0 0 0 0 0 0 0 0 40 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _