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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 12.12
Human Site: S186 Identified Species: 19.05
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 S186 L S G S A S P S R Y H G P A R
Chimpanzee Pan troglodytes XP_512373 411 46398 V153 E R R H R Q I V S W F A D H P
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 S186 L S G S A S P S R Y H G P A R
Dog Lupus familis XP_542069 473 52729 N185 P A G L A S P N R Y R G P A R
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 S186 M P G P A S P S R C R G P G R
Rat Rattus norvegicus NP_001094483 473 52805 S185 M P G P A S P S R Y R G P G R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 R178 P R P P S A S R N A P R G S G
Chicken Gallus gallus Q6PZ02 393 44512 E134 I A Q M G V G E G K S I G Q W
Frog Xenopus laevis Q68FJ9 469 52793 S173 M E P I R S S S P P S M P L S
Zebra Danio Brachydanio rerio Q6DG88 394 44435 G136 A Q M G V G E G K S I G Q W Y
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 F354 S S S K S S P F S I H A L V R
Honey Bee Apis mellifera XP_393739 477 54008 D206 K T E Q Q K L D E Y N H R L I
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 N437 H F L G R E W N I Y K P Q T Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 M171 S D V N W G C M I R S S Q M L
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 A242 L A N A I L I A R L G R E W R
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 0 100 66.6 N.A. 60 66.6 N.A. 0 0 20 6.6 N.A. 33.3 6.6 N.A. 6.6
P-Site Similarity: 100 6.6 100 80 N.A. 66.6 73.3 N.A. 20 13.3 26.6 13.3 N.A. 40 20 N.A. 20
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 20
P-Site Similarity: N.A. N.A. N.A. 6.6 N.A. 40
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 20 0 7 34 7 0 7 0 7 0 14 0 20 0 % A
% Cys: 0 0 0 0 0 0 7 0 0 7 0 0 0 0 0 % C
% Asp: 0 7 0 0 0 0 0 7 0 0 0 0 7 0 0 % D
% Glu: 7 7 7 0 0 7 7 7 7 0 0 0 7 0 0 % E
% Phe: 0 7 0 0 0 0 0 7 0 0 7 0 0 0 0 % F
% Gly: 0 0 34 14 7 14 7 7 7 0 7 40 14 14 7 % G
% His: 7 0 0 7 0 0 0 0 0 0 20 7 0 7 0 % H
% Ile: 7 0 0 7 7 0 14 0 14 7 7 7 0 0 7 % I
% Lys: 7 0 0 7 0 7 0 0 7 7 7 0 0 0 0 % K
% Leu: 20 0 7 7 0 7 7 0 0 7 0 0 7 14 7 % L
% Met: 20 0 7 7 0 0 0 7 0 0 0 7 0 7 0 % M
% Asn: 0 0 7 7 0 0 0 14 7 0 7 0 0 0 0 % N
% Pro: 14 14 14 20 0 0 40 0 7 7 7 7 40 0 7 % P
% Gln: 0 7 7 7 7 7 0 0 0 0 0 0 20 7 7 % Q
% Arg: 0 14 7 0 20 0 0 7 40 7 20 14 7 0 47 % R
% Ser: 14 20 7 14 14 47 14 34 14 7 20 7 0 7 7 % S
% Thr: 0 7 0 0 0 0 0 0 0 0 0 0 0 7 0 % T
% Val: 0 0 7 0 7 7 0 7 0 0 0 0 0 7 0 % V
% Trp: 0 0 0 0 7 0 7 0 0 7 0 0 0 14 7 % W
% Tyr: 0 0 0 0 0 0 0 0 0 40 0 0 0 0 7 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _