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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 22.73
Human Site: S264 Identified Species: 35.71
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 S264 R K A V E S C S D V T R L V V
Chimpanzee Pan troglodytes XP_512373 411 46398 S211 T R L V V Y V S Q D C T V Y K
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 S264 R K A V E S C S E V T R L V V
Dog Lupus familis XP_542069 473 52729 S263 R K A V E S C S E I T R L V V
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 S264 R K A V E S C S E V S R L V V
Rat Rattus norvegicus NP_001094483 473 52805 S263 R K A V E S C S E V T R L V V
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 R249 R K A V E E V R H P D L Q G L
Chicken Gallus gallus Q6PZ02 393 44512 A192 N F S C A G A A A C P A V E A
Frog Xenopus laevis Q68FJ9 469 52793 S259 K K A I E T S S E V P E L S V
Zebra Danio Brachydanio rerio Q6DG88 394 44435 C194 L D F D R G A C A V S E E P R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 Q418 Y L Q D I E D Q C S I P E P A
Honey Bee Apis mellifera XP_393739 477 54008 V264 S Q A V E N A V E R H P A F N
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 P508 D S A H E L N P L L G E V C I
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 G231 I I A G A S Y G L A A G S W V
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 A306 A R C I Q A L A D E K Q S G L
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 13.3 93.3 86.6 N.A. 86.6 93.3 N.A. 33.3 0 46.6 6.6 N.A. 0 20 N.A. 13.3
P-Site Similarity: 100 26.6 100 100 N.A. 100 100 N.A. 40 20 73.3 13.3 N.A. 0 40 N.A. 33.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 20 N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. 20 N.A. 53.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 0 67 0 14 7 20 14 14 7 7 7 7 0 14 % A
% Cys: 0 0 7 7 0 0 34 7 7 7 7 0 0 7 0 % C
% Asp: 7 7 0 14 0 0 7 0 14 7 7 0 0 0 0 % D
% Glu: 0 0 0 0 60 14 0 0 40 7 0 20 14 7 0 % E
% Phe: 0 7 7 0 0 0 0 0 0 0 0 0 0 7 0 % F
% Gly: 0 0 0 7 0 14 0 7 0 0 7 7 0 14 0 % G
% His: 0 0 0 7 0 0 0 0 7 0 7 0 0 0 0 % H
% Ile: 7 7 0 14 7 0 0 0 0 7 7 0 0 0 7 % I
% Lys: 7 47 0 0 0 0 0 0 0 0 7 0 0 0 7 % K
% Leu: 7 7 7 0 0 7 7 0 14 7 0 7 40 0 14 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 7 0 0 0 0 7 7 0 0 0 0 0 0 0 7 % N
% Pro: 0 0 0 0 0 0 0 7 0 7 14 14 0 14 0 % P
% Gln: 0 7 7 0 7 0 0 7 7 0 0 7 7 0 0 % Q
% Arg: 40 14 0 0 7 0 0 7 0 7 0 34 0 0 7 % R
% Ser: 7 7 7 0 0 40 7 47 0 7 14 0 14 7 0 % S
% Thr: 7 0 0 0 0 7 0 0 0 0 27 7 0 0 0 % T
% Val: 0 0 0 54 7 0 14 7 0 40 0 0 20 34 47 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 % W
% Tyr: 7 0 0 0 0 7 7 0 0 0 0 0 0 7 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _