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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 15.76
Human Site: S417 Identified Species: 24.76
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.21
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 S417 S E L T R V L S S S S A T E R
Chimpanzee Pan troglodytes XP_512373 411 46398 S355 E L T R V L S S S S A T E R Y
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 G417 S E L T R V L G S S S A T E R
Dog Lupus familis XP_542069 473 52729 S416 S E L T R V L S S S S A T E R
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 S417 S E L M R I L S S S S V T E R
Rat Rattus norvegicus NP_001094483 473 52805 S416 S E L M R I L S S S S V T E R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 E395 C R N V Q D F E R A S E E I T
Chicken Gallus gallus Q6PZ02 393 44512 K337 N D W C H Q I K K L S L V R G
Frog Xenopus laevis Q68FJ9 469 52793 E403 A F Y A K N S E D F G K L C D
Zebra Danio Brachydanio rerio Q6DG88 394 44435 I338 F D D W C A Q I R K V S N C R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 H571 E S V Q L Y L H P M R C A S G
Honey Bee Apis mellifera XP_393739 477 54008 V419 E I A P S Y L V P E D E K I D
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 S661 K E L P T V V S P L G S H S S
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 D380 T V N K E T P D V D T S S Y H
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 E450 I G F L I K D E D D W D T W K
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 20 93.3 100 N.A. 80 80 N.A. 6.6 6.6 0 6.6 N.A. 6.6 6.6 N.A. 26.6
P-Site Similarity: 100 33.3 93.3 100 N.A. 86.6 86.6 N.A. 20 26.6 13.3 20 N.A. 13.3 6.6 N.A. 40
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 0 7 7 0 7 0 0 0 7 7 20 7 0 0 % A
% Cys: 7 0 0 7 7 0 0 0 0 0 0 7 0 14 0 % C
% Asp: 0 14 7 0 0 7 7 7 14 14 7 7 0 0 14 % D
% Glu: 20 40 0 0 7 0 0 20 0 7 0 14 14 34 0 % E
% Phe: 7 7 7 0 0 0 7 0 0 7 0 0 0 0 0 % F
% Gly: 0 7 0 0 0 0 0 7 0 0 14 0 0 0 14 % G
% His: 0 0 0 0 7 0 0 7 0 0 0 0 7 0 7 % H
% Ile: 7 7 0 0 7 14 7 7 0 0 0 0 0 14 0 % I
% Lys: 7 0 0 7 7 7 0 7 7 7 0 7 7 0 7 % K
% Leu: 0 7 40 7 7 7 47 0 0 14 0 7 7 0 0 % L
% Met: 0 0 0 14 0 0 0 0 0 7 0 0 0 0 0 % M
% Asn: 7 0 14 0 0 7 0 0 0 0 0 0 7 0 0 % N
% Pro: 0 0 0 14 0 0 7 0 20 0 0 0 0 0 0 % P
% Gln: 0 0 0 7 7 7 7 0 0 0 0 0 0 0 0 % Q
% Arg: 0 7 0 7 34 0 0 0 14 0 7 0 0 14 40 % R
% Ser: 34 7 0 0 7 0 14 40 40 40 47 20 7 14 7 % S
% Thr: 7 0 7 20 7 7 0 0 0 0 7 7 40 0 7 % T
% Val: 0 7 7 7 7 27 7 7 7 0 7 14 7 0 0 % V
% Trp: 0 0 7 7 0 0 0 0 0 0 7 0 0 7 0 % W
% Tyr: 0 0 7 0 0 14 0 0 0 0 0 0 0 7 7 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _