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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 14.85
Human Site: S42 Identified Species: 23.33
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 S42 D P N G L G P S G A S G P A L
Chimpanzee Pan troglodytes XP_512373 411 46398 P15 G R C P Y V S P G W V V K S R
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 S42 D P N G L G P S G A S G L A L
Dog Lupus familis XP_542069 473 52729 S41 D P N G P R P S G A S G P A L
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 S42 D P S N L G P S G S G V A A L
Rat Rattus norvegicus NP_001094483 473 52805 S42 D P S N L G P S G S G V A A L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 R37 K T K T Y F S R N S P V L L L
Chicken Gallus gallus Q6PZ02 393 44512
Frog Xenopus laevis Q68FJ9 469 52793 E31 S T Q S G G H E Q R K M G H Q
Zebra Danio Brachydanio rerio Q6DG88 394 44435
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 C123 G G N S G P S C G H V E Q H N
Honey Bee Apis mellifera XP_393739 477 54008 L37 D R V P N I Q L T G T N N G L
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 N297 R S T S F D A N D E V E D E T
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 G32 S P L V S D S G P S D N K S K
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 G80 T L D I K Q Y G S P L S S S S
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 6.6 93.3 86.6 N.A. 60 60 N.A. 6.6 0 6.6 0 N.A. 13.3 13.3 N.A. 0
P-Site Similarity: 100 13.3 93.3 86.6 N.A. 73.3 73.3 N.A. 13.3 0 6.6 0 N.A. 13.3 20 N.A. 6.6
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 6.6 N.A. 0
P-Site Similarity: N.A. N.A. N.A. 20 N.A. 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 7 0 0 20 0 0 14 34 0 % A
% Cys: 0 0 7 0 0 0 0 7 0 0 0 0 0 0 0 % C
% Asp: 40 0 7 0 0 14 0 0 7 0 7 0 7 0 0 % D
% Glu: 0 0 0 0 0 0 0 7 0 7 0 14 0 7 0 % E
% Phe: 0 0 0 0 7 7 0 0 0 0 0 0 0 0 0 % F
% Gly: 14 7 0 20 14 34 0 14 47 7 14 20 7 7 0 % G
% His: 0 0 0 0 0 0 7 0 0 7 0 0 0 14 0 % H
% Ile: 0 0 0 7 0 7 0 0 0 0 0 0 0 0 0 % I
% Lys: 7 0 7 0 7 0 0 0 0 0 7 0 14 0 7 % K
% Leu: 0 7 7 0 27 0 0 7 0 0 7 0 14 7 47 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 % M
% Asn: 0 0 27 14 7 0 0 7 7 0 0 14 7 0 7 % N
% Pro: 0 40 0 14 7 7 34 7 7 7 7 0 14 0 0 % P
% Gln: 0 0 7 0 0 7 7 0 7 0 0 0 7 0 7 % Q
% Arg: 7 14 0 0 0 7 0 7 0 7 0 0 0 0 7 % R
% Ser: 14 7 14 20 7 0 27 34 7 27 20 7 7 20 7 % S
% Thr: 7 14 7 7 0 0 0 0 7 0 7 0 0 0 7 % T
% Val: 0 0 7 7 0 7 0 0 0 0 20 27 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % W
% Tyr: 0 0 0 0 14 0 7 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _