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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 20
Human Site: S445 Identified Species: 31.43
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 S445 H S L D D L C S Q L A Q P T L
Chimpanzee Pan troglodytes XP_512373 411 46398 Q383 S L D D L C S Q L A Q P T L R
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 S445 H S L D D L C S Q L S Q P T L
Dog Lupus familis XP_542069 473 52729 S444 H S L D D L C S Q L S Q P T L
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 T445 H S L D A L C T Q L S Q P T L
Rat Rattus norvegicus NP_001094483 473 52805 T444 H S L D A L C T Q L S Q P T L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 S423 F T F V K G H S R D Y D F A S
Chicken Gallus gallus Q6PZ02 393 44512 L365 H F S N P D V L N L T P D S S
Frog Xenopus laevis Q68FJ9 469 52793 S431 K Y P I F S I S E G Q A Q E Y
Zebra Danio Brachydanio rerio Q6DG88 394 44435 L366 H L I T A D V L N L T P D F S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 E599 P Q S S H Q T E Q T E M N Y P
Honey Bee Apis mellifera XP_393739 477 54008 A447 L H Q K I E I A E N I I P S T
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 N689 D W Q N K N T N T N R P E R Y
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 Y408 P S L A L G F Y C R D K D D F
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 R478 V S P Y D P A R G Q G G G R A
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 6.6 93.3 93.3 N.A. 80 80 N.A. 6.6 13.3 6.6 13.3 N.A. 6.6 6.6 N.A. 0
P-Site Similarity: 100 6.6 100 100 N.A. 93.3 93.3 N.A. 20 26.6 13.3 20 N.A. 6.6 26.6 N.A. 13.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 13.3 N.A. 13.3
P-Site Similarity: N.A. N.A. N.A. 20 N.A. 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 7 20 0 7 7 0 7 7 7 0 7 7 % A
% Cys: 0 0 0 0 0 7 34 0 7 0 0 0 0 0 0 % C
% Asp: 7 0 7 40 27 14 0 0 0 7 7 7 20 7 0 % D
% Glu: 0 0 0 0 0 7 0 7 14 0 7 0 7 7 0 % E
% Phe: 7 7 7 0 7 0 7 0 0 0 0 0 7 7 7 % F
% Gly: 0 0 0 0 0 14 0 0 7 7 7 7 7 0 0 % G
% His: 47 7 0 0 7 0 7 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 7 7 7 0 14 0 0 0 7 7 0 0 0 % I
% Lys: 7 0 0 7 14 0 0 0 0 0 0 7 0 0 0 % K
% Leu: 7 14 40 0 14 34 0 14 7 47 0 0 0 7 34 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 % M
% Asn: 0 0 0 14 0 7 0 7 14 14 0 0 7 0 0 % N
% Pro: 14 0 14 0 7 7 0 0 0 0 0 27 40 0 7 % P
% Gln: 0 7 14 0 0 7 0 7 40 7 14 34 7 0 0 % Q
% Arg: 0 0 0 0 0 0 0 7 7 7 7 0 0 14 7 % R
% Ser: 7 47 14 7 0 7 7 34 0 0 27 0 0 14 20 % S
% Thr: 0 7 0 7 0 0 14 14 7 7 14 0 7 34 7 % T
% Val: 7 0 0 7 0 0 14 0 0 0 0 0 0 0 0 % V
% Trp: 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 7 0 7 0 0 0 7 0 0 7 0 0 7 14 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _