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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 16.67
Human Site: S51 Identified Species: 26.19
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 S51 A S G P A L G S P G A G P S E
Chimpanzee Pan troglodytes XP_512373 411 46398 S24 W V V K S R T S F S K I S S I
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 S51 A S G L A L G S P G A G Q S E
Dog Lupus familis XP_542069 473 52729 S50 A S G P A L G S P A A A P G E
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 S51 S G V A A L G S S G T D P A E
Rat Rattus norvegicus NP_001094483 473 52805 S51 S G V A A L G S T G T D P A E
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 K46 S P V L L L G K C Y H F K S E
Chicken Gallus gallus Q6PZ02 393 44512
Frog Xenopus laevis Q68FJ9 469 52793 A40 R K M G H Q D A T L D G E A D
Zebra Danio Brachydanio rerio Q6DG88 394 44435
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 E132 H V E Q H N E E P G D L S A N
Honey Bee Apis mellifera XP_393739 477 54008 M46 G T N N G L S M D D S N V V T
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 T306 E V E D E T N T A S D E A G D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 T41 S D N K S K F T L W S N V F T
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 S89 P L S S S S L S Q L T A D T P
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 13.3 86.6 80 N.A. 46.6 46.6 N.A. 26.6 0 6.6 0 N.A. 13.3 6.6 N.A. 0
P-Site Similarity: 100 20 86.6 80 N.A. 60 60 N.A. 33.3 0 26.6 0 N.A. 20 20 N.A. 13.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. 20
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 20 0 0 14 34 0 0 7 7 7 20 14 7 27 0 % A
% Cys: 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 % C
% Asp: 0 7 0 7 0 0 7 0 7 7 20 14 7 0 14 % D
% Glu: 7 0 14 0 7 0 7 7 0 0 0 7 7 0 40 % E
% Phe: 0 0 0 0 0 0 7 0 7 0 0 7 0 7 0 % F
% Gly: 7 14 20 7 7 0 40 0 0 34 0 20 0 14 0 % G
% His: 7 0 0 0 14 0 0 0 0 0 7 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 7 % I
% Lys: 0 7 0 14 0 7 0 7 0 0 7 0 7 0 0 % K
% Leu: 0 7 0 14 7 47 7 0 7 14 0 7 0 0 0 % L
% Met: 0 0 7 0 0 0 0 7 0 0 0 0 0 0 0 % M
% Asn: 0 0 14 7 0 7 7 0 0 0 0 14 0 0 7 % N
% Pro: 7 7 0 14 0 0 0 0 27 0 0 0 27 0 7 % P
% Gln: 0 0 0 7 0 7 0 0 7 0 0 0 7 0 0 % Q
% Arg: 7 0 0 0 0 7 0 0 0 0 0 0 0 0 0 % R
% Ser: 27 20 7 7 20 7 7 47 7 14 14 0 14 27 0 % S
% Thr: 0 7 0 0 0 7 7 14 14 0 20 0 0 7 14 % T
% Val: 0 20 27 0 0 0 0 0 0 0 0 0 14 7 0 % V
% Trp: 7 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _