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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 12.42
Human Site: T167 Identified Species: 19.52
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 T167 H F L P R D W T W A E G M G L
Chimpanzee Pan troglodytes XP_512373 411 46398 P134 G P A R W M P P R W A Q G A P
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 T167 H F L P R D W T W A E G T G L
Dog Lupus familis XP_542069 473 52729 T166 H F L P R D W T W A E G P G L
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 R167 H F L P R D W R W V E G T G L
Rat Rattus norvegicus NP_001094483 473 52805 R166 H F L P R D W R W V E G T G L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 A159 H T V K K L T A S F E A S W V
Chicken Gallus gallus Q6PZ02 393 44512 I115 F S V L N A F I D K K D S Y Y
Frog Xenopus laevis Q68FJ9 469 52793 T154 H L L S R E W T W S E A L Y R
Zebra Danio Brachydanio rerio Q6DG88 394 44435 D117 S I L N A F I D K K D S Y Y S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 N335 L H S T Y E D N M H K K I V K
Honey Bee Apis mellifera XP_393739 477 54008 G187 A L V C H F L G R E W R W Q P
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 S418 G W G C M L R S G Q M M L A H
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 K152 K I L M T Y R K G F E P F R D
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 G223 T G F S S D T G W G C M I R S
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 0 93.3 93.3 N.A. 80 80 N.A. 13.3 0 46.6 6.6 N.A. 0 0 N.A. 0
P-Site Similarity: 100 0 93.3 93.3 N.A. 80 80 N.A. 33.3 20 66.6 13.3 N.A. 20 6.6 N.A. 20
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 13.3 N.A. 13.3
P-Site Similarity: N.A. N.A. N.A. 13.3 N.A. 20
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 0 7 0 7 7 0 7 0 20 7 14 0 14 0 % A
% Cys: 0 0 0 14 0 0 0 0 0 0 7 0 0 0 0 % C
% Asp: 0 0 0 0 0 40 7 7 7 0 7 7 0 0 7 % D
% Glu: 0 0 0 0 0 14 0 0 0 7 54 0 0 0 0 % E
% Phe: 7 34 7 0 0 14 7 0 0 14 0 0 7 0 0 % F
% Gly: 14 7 7 0 0 0 0 14 14 7 0 34 7 34 0 % G
% His: 47 7 0 0 7 0 0 0 0 7 0 0 0 0 7 % H
% Ile: 0 14 0 0 0 0 7 7 0 0 0 0 14 0 0 % I
% Lys: 7 0 0 7 7 0 0 7 7 14 14 7 0 0 7 % K
% Leu: 7 14 54 7 0 14 7 0 0 0 0 0 14 0 34 % L
% Met: 0 0 0 7 7 7 0 0 7 0 7 14 7 0 0 % M
% Asn: 0 0 0 7 7 0 0 7 0 0 0 0 0 0 0 % N
% Pro: 0 7 0 34 0 0 7 7 0 0 0 7 7 0 14 % P
% Gln: 0 0 0 0 0 0 0 0 0 7 0 7 0 7 0 % Q
% Arg: 0 0 0 7 40 0 14 14 14 0 0 7 0 14 7 % R
% Ser: 7 7 7 14 7 0 0 7 7 7 0 7 14 0 14 % S
% Thr: 7 7 0 7 7 0 14 27 0 0 0 0 20 0 0 % T
% Val: 0 0 20 0 0 0 0 0 0 14 0 0 0 7 7 % V
% Trp: 0 7 0 0 7 0 40 0 47 7 7 0 7 7 0 % W
% Tyr: 0 0 0 0 7 7 0 0 0 0 0 0 7 20 7 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _