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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 8.79
Human Site: T413 Identified Species: 13.81
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 T413 E T L C S E L T R V L S S S S
Chimpanzee Pan troglodytes XP_512373 411 46398 R351 T L C S E L T R V L S S S S A
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 T413 E T L C S E L T R V L G S S S
Dog Lupus familis XP_542069 473 52729 T412 E T L C S E L T R V L S S S S
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 M413 E T L C S E L M R I L S S S S
Rat Rattus norvegicus NP_001094483 473 52805 M412 E T L C S E L M R I L S S S S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 V391 V G F Y C R N V Q D F E R A S
Chicken Gallus gallus Q6PZ02 393 44512 C333 E E D F N D W C H Q I K K L S
Frog Xenopus laevis Q68FJ9 469 52793 A399 S C T F A F Y A K N S E D F G
Zebra Danio Brachydanio rerio Q6DG88 394 44435 W334 T E D D F D D W C A Q I R K V
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 Q567 D N F M E S V Q L Y L H P M R
Honey Bee Apis mellifera XP_393739 477 54008 P415 T N F M E I A P S Y L V P E D
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 P657 E Q L C K E L P T V V S P L G
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 K376 Q Q V V T V N K E T P D V D T
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 L446 P S M L I G F L I K D E D D W
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 20 93.3 100 N.A. 86.6 86.6 N.A. 6.6 13.3 0 0 N.A. 6.6 6.6 N.A. 46.6
P-Site Similarity: 100 33.3 93.3 100 N.A. 93.3 93.3 N.A. 20 33.3 13.3 6.6 N.A. 20 6.6 N.A. 53.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 0
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 7 0 7 7 0 7 0 0 0 7 7 % A
% Cys: 0 7 7 40 7 0 0 7 7 0 0 0 0 0 0 % C
% Asp: 7 0 14 7 0 14 7 0 0 7 7 7 14 14 7 % D
% Glu: 47 14 0 0 20 40 0 0 7 0 0 20 0 7 0 % E
% Phe: 0 0 20 14 7 7 7 0 0 0 7 0 0 7 0 % F
% Gly: 0 7 0 0 0 7 0 0 0 0 0 7 0 0 14 % G
% His: 0 0 0 0 0 0 0 0 7 0 0 7 0 0 0 % H
% Ile: 0 0 0 0 7 7 0 0 7 14 7 7 0 0 0 % I
% Lys: 0 0 0 0 7 0 0 7 7 7 0 7 7 7 0 % K
% Leu: 0 7 40 7 0 7 40 7 7 7 47 0 0 14 0 % L
% Met: 0 0 7 14 0 0 0 14 0 0 0 0 0 7 0 % M
% Asn: 0 14 0 0 7 0 14 0 0 7 0 0 0 0 0 % N
% Pro: 7 0 0 0 0 0 0 14 0 0 7 0 20 0 0 % P
% Gln: 7 14 0 0 0 0 0 7 7 7 7 0 0 0 0 % Q
% Arg: 0 0 0 0 0 7 0 7 34 0 0 0 14 0 7 % R
% Ser: 7 7 0 7 34 7 0 0 7 0 14 40 40 40 47 % S
% Thr: 20 34 7 0 7 0 7 20 7 7 0 0 0 0 7 % T
% Val: 7 0 7 7 0 7 7 7 7 27 7 7 7 0 7 % V
% Trp: 0 0 0 0 0 0 7 7 0 0 0 0 0 0 7 % W
% Tyr: 0 0 0 7 0 0 7 0 0 14 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _