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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 21.82
Human Site: Y101 Identified Species: 34.29
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 Y101 I H L C G R R Y R F E G E G D
Chimpanzee Pan troglodytes XP_512373 411 46398 T74 P L P G G C L T S D C G W G C
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 Y101 I H L C G R R Y R F E G E G D
Dog Lupus familis XP_542069 473 52729 Y100 V H L C G R R Y R F E G E G D
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 Y101 V H L C G R C Y H F E G E G D
Rat Rattus norvegicus NP_001094483 473 52805 Y101 V H L C G R C Y H F E G E G D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 P96 L T Y R E E F P P M G A S G L
Chicken Gallus gallus Q6PZ02 393 44512 K55 R L W F T Y R K N F P A I G G
Frog Xenopus laevis Q68FJ9 469 52793 F90 L L G E R Y F F R L D D E I E
Zebra Danio Brachydanio rerio Q6DG88 394 44435 N57 L W F T Y R K N F Q P I G G T
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 C182 P V W L L G R C Y H R R F T P
Honey Bee Apis mellifera XP_393739 477 54008 Y96 V C L L G K I Y R K K P E E F
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 Y356 I W F L G K C Y H Q R P E D P
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 R91 M A S G A I R R F Q E R V L G
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 G139 A P T G S Q L G S F D T V P D
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 20 100 93.3 N.A. 80 80 N.A. 6.6 20 13.3 13.3 N.A. 6.6 33.3 N.A. 26.6
P-Site Similarity: 100 20 100 100 N.A. 86.6 86.6 N.A. 13.3 20 40 26.6 N.A. 6.6 53.3 N.A. 33.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 13.3 N.A. 13.3
P-Site Similarity: N.A. N.A. N.A. 20 N.A. 26.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 7 0 0 7 0 0 0 0 0 0 14 0 0 0 % A
% Cys: 0 7 0 34 0 7 20 7 0 0 7 0 0 0 7 % C
% Asp: 0 0 0 0 0 0 0 0 0 7 14 7 0 7 40 % D
% Glu: 0 0 0 7 7 7 0 0 0 0 40 0 54 7 7 % E
% Phe: 0 0 14 7 0 0 14 7 14 47 0 0 7 0 7 % F
% Gly: 0 0 7 20 54 7 0 7 0 0 7 40 7 60 14 % G
% His: 0 34 0 0 0 0 0 0 20 7 0 0 0 0 0 % H
% Ile: 20 0 0 0 0 7 7 0 0 0 0 7 7 7 0 % I
% Lys: 0 0 0 0 0 14 7 7 0 7 7 0 0 0 0 % K
% Leu: 20 20 40 20 7 0 14 0 0 7 0 0 0 7 7 % L
% Met: 7 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 7 7 0 0 0 0 0 0 % N
% Pro: 14 7 7 0 0 0 0 7 7 0 14 14 0 7 14 % P
% Gln: 0 0 0 0 0 7 0 0 0 20 0 0 0 0 0 % Q
% Arg: 7 0 0 7 7 40 40 7 34 0 14 14 0 0 0 % R
% Ser: 0 0 7 0 7 0 0 0 14 0 0 0 7 0 0 % S
% Thr: 0 7 7 7 7 0 0 7 0 0 0 7 0 7 7 % T
% Val: 27 7 0 0 0 0 0 0 0 0 0 0 14 0 0 % V
% Trp: 0 14 14 0 0 0 0 0 0 0 0 0 7 0 0 % W
% Tyr: 0 0 7 0 7 14 0 47 7 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _