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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 25.76
Human Site: Y317 Identified Species: 40.48
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 Y317 G E T L N P V Y V P C V K E L
Chimpanzee Pan troglodytes XP_512373 411 46398 C264 C V K E L L R C E L C L G I M
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 Y317 G E T L N P V Y V P C V K E L
Dog Lupus familis XP_542069 473 52729 Y316 G E T L N P V Y V P C V K E L
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 Y317 G E T L N P V Y V P C V K E L
Rat Rattus norvegicus NP_001094483 473 52805 Y316 G E T L N P V Y V P C V K E L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 T302 V R L G G E R T N I D Y L E F
Chicken Gallus gallus Q6PZ02 393 44512 H245 A Y I E T L K H C F M M P Q S
Frog Xenopus laevis Q68FJ9 469 52793 T312 P V R L G G E T F N P V Y K H
Zebra Danio Brachydanio rerio Q6DG88 394 44435 Q247 A Y I E P L K Q C F M M P Q S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 Y471 S D K L N P V Y A H C L K L L
Honey Bee Apis mellifera XP_393739 477 54008 P317 L G A D K L N P V Y T S C L T
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 Y561 G D E V N P V Y I R P I Q S L
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 P284 D G E R G G A P I L C I E D A
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 V359 G I D K I N Q V Y E E A L I S
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 6.6 100 100 N.A. 100 100 N.A. 6.6 0 13.3 0 N.A. 53.3 6.6 N.A. 40
P-Site Similarity: 100 20 100 100 N.A. 100 100 N.A. 6.6 20 20 13.3 N.A. 66.6 6.6 N.A. 73.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 6.6 N.A. 6.6
P-Site Similarity: N.A. N.A. N.A. 33.3 N.A. 6.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 14 0 7 0 0 0 7 0 7 0 0 7 0 0 7 % A
% Cys: 7 0 0 0 0 0 0 7 14 0 54 0 7 0 0 % C
% Asp: 7 14 7 7 0 0 0 0 0 0 7 0 0 7 0 % D
% Glu: 0 34 14 20 0 7 7 0 7 7 7 0 7 40 0 % E
% Phe: 0 0 0 0 0 0 0 0 7 14 0 0 0 0 7 % F
% Gly: 47 14 0 7 20 14 0 0 0 0 0 0 7 0 0 % G
% His: 0 0 0 0 0 0 0 7 0 7 0 0 0 0 7 % H
% Ile: 0 7 14 0 7 0 0 0 14 7 0 14 0 14 0 % I
% Lys: 0 0 14 7 7 0 14 0 0 0 0 0 40 7 0 % K
% Leu: 7 0 7 47 7 27 0 0 0 14 0 14 14 14 47 % L
% Met: 0 0 0 0 0 0 0 0 0 0 14 14 0 0 7 % M
% Asn: 0 0 0 0 47 7 7 0 7 7 0 0 0 0 0 % N
% Pro: 7 0 0 0 7 47 0 14 0 34 14 0 14 0 0 % P
% Gln: 0 0 0 0 0 0 7 7 0 0 0 0 7 14 0 % Q
% Arg: 0 7 7 7 0 0 14 0 0 7 0 0 0 0 0 % R
% Ser: 7 0 0 0 0 0 0 0 0 0 0 7 0 7 20 % S
% Thr: 0 0 34 0 7 0 0 14 0 0 7 0 0 0 7 % T
% Val: 7 14 0 7 0 0 47 7 40 0 0 40 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 14 0 0 0 0 0 47 7 7 0 7 7 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _