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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4D All Species: 18.79
Human Site: Y354 Identified Species: 29.52
UniProt: Q86TL0 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q86TL0 NP_116274.3 474 52922 Y354 Y Q D D F L L Y L D P H Y C Q
Chimpanzee Pan troglodytes XP_512373 411 46398 L292 Q D D F L L Y L D P H Y C Q P
Rhesus Macaque Macaca mulatta XP_001101860 474 52893 Y354 Y Q D D F L L Y L D P H Y C Q
Dog Lupus familis XP_542069 473 52729 Y353 Y Q D D F L L Y L D P H Y C Q
Cat Felis silvestris
Mouse Mus musculus Q8BGV9 474 52892 Y354 Y Q D D F L L Y L D P H Y C Q
Rat Rattus norvegicus NP_001094483 473 52805 Y353 Y Q D D F L L Y L D P H Y C Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 Y332 G K P K Q S Y Y F A G F Q D D
Chicken Gallus gallus Q6PZ02 393 44512 L274 I G Y V G E E L I Y L D P H T
Frog Xenopus laevis Q68FJ9 469 52793 H340 I I G G K P K H S L Y F I G Y
Zebra Danio Brachydanio rerio Q6DG88 394 44435 E275 F I G F V G D E L I Y L D P H
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 H508 F Q E D R L I H L D P H Y C Q
Honey Bee Apis mellifera XP_393739 477 54008 N356 F Q E D K L I N L D P H Y C Q
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 H598 F Q E E K L I H L D P H Y C Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 G317 L L V P L V L G L D S V N P R
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 F387 R P S S S H Y F V G V Q G Q R
Conservation
Percent
Protein Identity: 100 84.8 98.5 94.3 N.A. 86.2 86.7 N.A. 44 26.5 53.3 27 N.A. 30.3 35 N.A. 30.8
Protein Similarity: 100 85.6 98.9 96.1 N.A. 90.9 91.1 N.A. 59.4 39.6 68.9 41.9 N.A. 44.4 49.2 N.A. 41.9
P-Site Identity: 100 13.3 100 100 N.A. 100 100 N.A. 6.6 0 0 6.6 N.A. 66.6 66.6 N.A. 60
P-Site Similarity: 100 20 100 100 N.A. 100 100 N.A. 13.3 6.6 6.6 13.3 N.A. 93.3 86.6 N.A. 93.3
Percent
Protein Identity: N.A. N.A. N.A. 27.4 N.A. 22.1
Protein Similarity: N.A. N.A. N.A. 42.4 N.A. 36.3
P-Site Identity: N.A. N.A. N.A. 20 N.A. 0
P-Site Similarity: N.A. N.A. N.A. 33.3 N.A. 20
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 7 54 0 % C
% Asp: 0 7 40 47 0 0 7 0 7 60 0 7 7 7 7 % D
% Glu: 0 0 20 7 0 7 7 7 0 0 0 0 0 0 0 % E
% Phe: 27 0 0 14 34 0 0 7 7 0 0 14 0 0 0 % F
% Gly: 7 7 14 7 7 7 0 7 0 7 7 0 7 7 0 % G
% His: 0 0 0 0 0 7 0 20 0 0 7 54 0 7 7 % H
% Ile: 14 14 0 0 0 0 20 0 7 7 0 0 7 0 0 % I
% Lys: 0 7 0 7 20 0 7 0 0 0 0 0 0 0 0 % K
% Leu: 7 7 0 0 14 60 40 14 67 7 7 7 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 7 0 0 0 0 7 0 0 % N
% Pro: 0 7 7 7 0 7 0 0 0 7 54 0 7 14 7 % P
% Gln: 7 54 0 0 7 0 0 0 0 0 0 7 7 14 54 % Q
% Arg: 7 0 0 0 7 0 0 0 0 0 0 0 0 0 14 % R
% Ser: 0 0 7 7 7 7 0 0 7 0 7 0 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 % T
% Val: 0 0 7 7 7 7 0 0 7 0 7 7 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 34 0 7 0 0 0 20 40 0 7 14 7 54 0 7 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _