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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: APLF All Species: 4.24
Human Site: S214 Identified Species: 11.67
UniProt: Q8IW19 Number Species: 8
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8IW19 NP_775816.1 511 56956 S214 G G N V I Q G S G K E E I C K
Chimpanzee Pan troglodytes XP_515525 303 34195 S33 R Q L I S S G S S E N T S A E
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_538525 510 57035 Q213 S E D N T V I Q G S R K E G I
Cat Felis silvestris
Mouse Mus musculus Q9D842 499 54950 G206 S L S T P A E G G D K D V I Q
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514287 555 61656 G242 T P T N A S T G V T K G K G K
Chicken Gallus gallus XP_419335 514 57457 S209 G G N R N K K S Q G R G K R H
Frog Xenopus laevis
Zebra Danio Brachydanio rerio NP_001099159 262 29285
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster A8JR14 255 27987
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_788055 674 74378 S323 D D D E D Y L S E D D K P A P
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 57.9 N.A. 72.2 N.A. 60 N.A. N.A. 41.2 44.7 N.A. 23.4 N.A. 21.3 N.A. N.A. 28.1
Protein Similarity: 100 58.9 N.A. 81.4 N.A. 74.7 N.A. N.A. 57.2 61.8 N.A. 33 N.A. 31.5 N.A. N.A. 43.7
P-Site Identity: 100 13.3 N.A. 6.6 N.A. 6.6 N.A. N.A. 6.6 26.6 N.A. 0 N.A. 0 N.A. N.A. 6.6
P-Site Similarity: 100 33.3 N.A. 20 N.A. 40 N.A. N.A. 13.3 33.3 N.A. 0 N.A. 0 N.A. N.A. 26.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 12 12 0 0 0 0 0 0 0 23 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 12 0 % C
% Asp: 12 12 23 0 12 0 0 0 0 23 12 12 0 0 0 % D
% Glu: 0 12 0 12 0 0 12 0 12 12 12 12 12 0 12 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 23 23 0 0 0 0 23 23 34 12 0 23 0 23 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 % H
% Ile: 0 0 0 12 12 0 12 0 0 0 0 0 12 12 12 % I
% Lys: 0 0 0 0 0 12 12 0 0 12 23 23 23 0 23 % K
% Leu: 0 12 12 0 0 0 12 0 0 0 0 0 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 23 23 12 0 0 0 0 0 12 0 0 0 0 % N
% Pro: 0 12 0 0 12 0 0 0 0 0 0 0 12 0 12 % P
% Gln: 0 12 0 0 0 12 0 12 12 0 0 0 0 0 12 % Q
% Arg: 12 0 0 12 0 0 0 0 0 0 23 0 0 12 0 % R
% Ser: 23 0 12 0 12 23 0 45 12 12 0 0 12 0 0 % S
% Thr: 12 0 12 12 12 0 12 0 0 12 0 12 0 0 0 % T
% Val: 0 0 0 12 0 12 0 0 12 0 0 0 12 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 12 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _