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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: RHOT1 All Species: 22.42
Human Site: T591 Identified Species: 35.24
UniProt: Q8IXI2 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q8IXI2 NP_001028738.1 618 70784 T591 Q A D L K S S T F W L R A S F
Chimpanzee Pan troglodytes XP_511399 695 80129 D595 Y R D R L S R D M G H T D R I
Rhesus Macaque Macaca mulatta XP_001109912 830 94570 D730 Y R D R L S R D M G H T D R I
Dog Lupus familis XP_867966 631 72105 T604 Q A D L K S S T F W L R A S F
Cat Felis silvestris
Mouse Mus musculus Q8BG51 631 72223 T604 Q A D L K S S T F W L R A S F
Rat Rattus norvegicus Q7TSA0 622 69135 P592 H T E L H P T P F W L R G V L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511136 687 78991 K593 Q N M D E M D K L K H E K K E
Chicken Gallus gallus Q5ZM73 619 70876 T592 Q A D L K S S T F W L R A S F
Frog Xenopus laevis NP_001121318 618 70967 T591 Q A E L K N S T F W L R A S F
Zebra Danio Brachydanio rerio Q6NVC5 619 71179 T592 Q A D L K N S T F W L R A S V
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q8IMX7 652 73757 L622 L M T E D P K L W L K A G L G
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q94263 625 70204 L598 H L R R V F Y L N D S N L L S
Sea Urchin Strong. purpuratus XP_791124 583 66070 P557 F C T H Y G L P P P Q T F S S
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P39722 662 75132 A624 P G L P E E T A A K D V D Y R
Red Bread Mold Neurospora crassa Q7RZA2 629 70228 D599 R S E E P P A D R A S L Y M A
Conservation
Percent
Protein Identity: 100 87 74.3 96.6 N.A. 95.8 58 N.A. 85.5 93.8 85.5 85.9 N.A. 50.6 N.A. 44 59.5
Protein Similarity: 100 88.1 74.4 97.4 N.A. 97.6 75 N.A. 87.9 97.7 94.1 94.8 N.A. 68.8 N.A. 64.1 72.9
P-Site Identity: 100 13.3 13.3 100 N.A. 100 33.3 N.A. 6.6 100 86.6 86.6 N.A. 0 N.A. 0 6.6
P-Site Similarity: 100 13.3 13.3 100 N.A. 100 46.6 N.A. 13.3 100 100 93.3 N.A. 6.6 N.A. 0 6.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. 31.1 34.5
Protein Similarity: N.A. N.A. N.A. N.A. 49.8 53.9
P-Site Identity: N.A. N.A. N.A. N.A. 0 0
P-Site Similarity: N.A. N.A. N.A. N.A. 13.3 26.6
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 40 0 0 0 0 7 7 7 7 0 7 40 0 7 % A
% Cys: 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 47 7 7 0 7 20 0 7 7 0 20 0 0 % D
% Glu: 0 0 20 14 14 7 0 0 0 0 0 7 0 0 7 % E
% Phe: 7 0 0 0 0 7 0 0 47 0 0 0 7 0 34 % F
% Gly: 0 7 0 0 0 7 0 0 0 14 0 0 14 0 7 % G
% His: 14 0 0 7 7 0 0 0 0 0 20 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 % I
% Lys: 0 0 0 0 40 0 7 7 0 14 7 0 7 7 0 % K
% Leu: 7 7 7 47 14 0 7 14 7 7 47 7 7 14 7 % L
% Met: 0 7 7 0 0 7 0 0 14 0 0 0 0 7 0 % M
% Asn: 0 7 0 0 0 14 0 0 7 0 0 7 0 0 0 % N
% Pro: 7 0 0 7 7 20 0 14 7 7 0 0 0 0 0 % P
% Gln: 47 0 0 0 0 0 0 0 0 0 7 0 0 0 0 % Q
% Arg: 7 14 7 20 0 0 14 0 7 0 0 47 0 14 7 % R
% Ser: 0 7 0 0 0 40 40 0 0 0 14 0 0 47 14 % S
% Thr: 0 7 14 0 0 0 14 40 0 0 0 20 0 0 0 % T
% Val: 0 0 0 0 7 0 0 0 0 0 0 7 0 7 7 % V
% Trp: 0 0 0 0 0 0 0 0 7 47 0 0 0 0 0 % W
% Tyr: 14 0 0 0 7 0 7 0 0 0 0 0 7 7 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _